SPHK1 Recombinant Rabbit Monoclonal Antibody [JA31-14]
cat.: ET1704-76
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JA31-14 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 43 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human SPHK1 aa 1-200 / 384. |
| Positive control: | HepG2 cell lysate, HeLa cell lysate, MCF7 cell lysate, RAW264.7 cell lysate, Mouse lung tissue lysate. |
| Subcellular location: | Cytoplasm. Nucleus. Membrane. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: Q9NYA1 Human | Q8CI15 Mouse |
| Alternative names: | SK 1 SK1 Sphingosine kinase 1 SPHK 1 SPHK Sphk1 SPHK1_HUMAN SPK SPK 1 |
Images
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Fig1:
Western blot analysis of SPHK1 on different lysates with Rabbit anti-SPHK1 antibody (ET1704-76) at 1/5,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: MCF7 cell lysate (20 µg/Lane) Lane 4: RAW264.7 cell lysate (20 µg/Lane) Lane 5: Mouse lung tissue lysate (30 µg/Lane) Lane 6: Mouse skeletal muscle tissue lysate (negative) (30 µg/Lane) Predicted band size: 43 kDa Observed band size: 43-51 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-76) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.