OGG1 Recombinant Rabbit Monoclonal Antibody [JA18-13]
cat.: ET1704-79
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JA18-13 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 39 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human OGG1 aa 296-345 / 345. |
| Positive control: | Hep G2 (Human hepatocellular carcinoma cell) cell lysate, A549 (Human lung adenocarcinoma cell) cell lysate, 293T (Human embryonic kidney cell) cell lysate, HeLa (Human cervical adenocarcinoma cell) cell lysate, Raji (Human Burkitt's lymphoma cell) cell lysate, Daudi (Human Burkitt's lymphoma cell) cell lysate, human kidney tissue. |
| Subcellular location: | Nucleus, nucleoplasm, Nucleus speckle, Nucleus matrix; Mitochondrion. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:50-1:200 |
| Uniprot #: | SwissProt: O15527 Human |
| Alternative names: | 8 hydroxyguanine DNA glycosylase 8 oxoguanine DNA glycosylase 1 8 oxoguanine DNA glycosylase AP lyase DNA (apurinic or apyrimidinic site) lyase DNA apurinic or apyrimidinic site lyase DNA lyase DNA-(apurinic or apyrimidinic site) lyase HMMH HOGG 1 HOGG1 MMH MUTM N glycosylase Ogg 1 Ogg1 OGG1_HUMAN OGH 1 OGH1 |
Images
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Fig1:
Western blot analysis of OGG1 on different lysates with Rabbit anti-OGG1 antibody (ET1704-79) at 1/5,000 dilution. Lane 1: Hep G2 (Human hepatocellular carcinoma cell) cell lysate Lane 2: A549 (Human lung adenocarcinoma cell) cell lysate Lane 3: 293T (Human embryonic kidney cell) cell lysate Lane 4: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 5: Raji (Human Burkitt's lymphoma cell) cell lysate Lane 6: Daudi (Human Burkitt's lymphoma cell) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute 2 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1704-79, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 39 kDa Observed band size: 35 kDa |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-OGG1 antibody (ET1704-79) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-79) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.