OGG1 Recombinant Rabbit Monoclonal Antibody [JA18-13]
cat.: ET1704-79
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JA18-13 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 39 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human OGG1 aa 296-345 / 345. |
| Positive control: | Human kidney tissue, Daudi cell lysates. |
| Subcellular location: | Mitochondrion. Nucleus |
| Recommended Dilutions:
WB IHC-P |
1:500 1:50-1:200 |
| Uniprot #: | SwissProt: O15527 Human |
| Alternative names: | 8 hydroxyguanine DNA glycosylase 8 oxoguanine DNA glycosylase 1 8 oxoguanine DNA glycosylase AP lyase DNA (apurinic or apyrimidinic site) lyase DNA apurinic or apyrimidinic site lyase DNA lyase DNA-(apurinic or apyrimidinic site) lyase HMMH HOGG 1 HOGG1 MMH MUTM N glycosylase Ogg 1 Ogg1 OGG1_HUMAN OGH 1 OGH1 |
Images
|
Fig1:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-OGG1 antibody (ET1704-79) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-79) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Western blot analysis of OGG1 on Daudi cell lysates with Rabbit anti-OGG1 antibody (ET1704-79) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 39 kDa Observed band size: 39 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-79) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.