NMDAR2A Recombinant Rabbit Monoclonal Antibody [JA31-20]
cat.: ET1704-80
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Cynomolgus monkey, Pig |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JA31-20 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 165 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human NMDAR2A aa 1356-1405 / 1464. |
| Positive control: | Rat brain tissue lysate, rat brain tissue, rat skin tissue, human brain tissue, mouse small intestine tissue. |
| Subcellular location: | Cell membrane, postsynaptic cell membrane, dendritic spine, synapse, Cytoplasmic vesicle membrane. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:500 1:50-1:100 1:200 |
| Uniprot #: | SwissProt: Q12879 Human | P35436 Mouse | Q00959 Rat |
| Alternative names: | EPND FESD GluN2A Glutamate [NMDA] receptor subunit epsilon-1 Glutamate receptor Glutamate receptor ionotropic N methyl D aspartate 2A GRIN 2A GRIN2A hNR2A LKS N methyl D aspartate receptor channel, subunit epsilon 1 N Methyl D Aspartate Receptor Subtype 2A N methyl D aspartate receptor subunit 2A N-methyl D-aspartate receptor subtype 2A NMDA receptor subtype 2A NMDAR 2A NMDAR2A NMDE1_HUMAN NR2A OTTHUMP00000160135 OTTHUMP00000174531 |
Images
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Fig1: Western blot analysis of NMDAR2A on mouse hippocampus tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-80, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-NMDAR2A antibody (ET1704-80) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-80) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded rat skin tissue using anti-NMDAR2A antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-NMDAR2A antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-NMDAR2A antibody. Counter stained with hematoxylin. |
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Fig6:
Immunofluorescence analysis of paraffin-embedded human brain tissue labeling NMDAR2A with Rabbit anti-NMDAR2A antibody (ET1704-80) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1704-80, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.