TXNRD1 Recombinant Rabbit Monoclonal Antibody [JA11-32]
cat.: ET1704-81
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | JA11-32 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 55 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein with Human TXNRD1 aa 1-649 / 649. |
| Positive control: | Hela cell lysate, human liver tissue lysate, HUVEC, human kidney tissue, mouse testis tissue, SH-SY5Y. |
| Subcellular location: | Cytoplasm. Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:2,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q16881 Human | Q9JMH6 Mouse | O89049 Rat |
| Alternative names: | cytoplasmic Gene associated with retinoic and IFN-induced mortality 12 protein Gene associated with retinoic and interferon-induced mortality 12 protein Gene associated with retinoid IFN induced mortality 12 protein GRIM 12 GRIM-12 GRIM12 KDRF KM 102 derived reductase like factor KM-102-derived reductase-like factor MGC9145 Oxidoreductase Thioredoxin reductase 1 Thioredoxin reductase 1 cytoplasmic Thioredoxin reductase GRIM 12 Thioredoxin reductase TR1 TR 1 TR TR1 TRXR 1 TRXR1 TRXR1_HUMAN TXNR TXNRD 1 Txnrd1 |
Images
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Fig1:
Western blot analysis of TXNRD1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-81, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: Human liver tissue lysate |
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Fig2: ICC staining of TXNRD1 in HUVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-81, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TXNRD1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-81, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-TXNRD1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-81, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Flow cytometric analysis of TXNRD1 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1704-81, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.