Factor H Recombinant Rabbit Monoclonal Antibody [JA52-13]
cat.: ET1704-83
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JA52-13 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 140 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Factor H aa 131-180 / 1231. |
| Positive control: | Human kidney tissue lysates, Hela, HepG2, human liver tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:2,000 1:50-1:100 1:50-1:100 |
| Uniprot #: | SwissProt: P08603 Human |
| Alternative names: | adrenomedullin binding protein age related maculopathy susceptibility 1 AHUS 1 AHUS1 AMBP 1 AMBP1 ARMD 4 ARMD4 ARMS 1 ARMS1 beta 1 H globulin beta 1H beta1H CFAH_HUMAN CFH CFHL 3 CFHL3 Complement factor H complement factor H, isoform b Factor H factor H like 1 FH FHL 1 FHL1 H factor 1 (complement) H factor 1 H factor 2 (complement) HF 1 HF 2 HF HF1 HF2 HUS MGC88246 |
Images
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Fig1: Western blot analysis of Factor H on human kidney tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-83, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig2: ICC staining of Factor H in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-83, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
|
Fig3: ICC staining of Factor H in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-83, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
|
Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Factor H antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-83, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.