| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | JA13-26 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 43 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human MEIS1 aa 328-377 / 390. |
| Positive control: | HeLa cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:500-1:2,000 |
| Uniprot #: | SwissProt: O00470 Human | Q60954 Mouse Entrez Gene: 686117 Rat |
| Alternative names: | Homeo box protein Meis1 Homeobox protein Meis1 Leukemogenic homolog protein MEIS 1 Meis homeo box 1 Meis homeobox 1 Meis1 Meis1 mouse homolog Meis1 myeloid ecotropic viral integration site 1 homolog Meis1 myeloid ecotropic viral integration site 1 homolog mouse MEIS1 protein MEIS1_HUMAN MGC43380 Myeloid ecotropic viral integration site 1 homolog WUGSC:H NH0444B04.1 |
|
Fig1:
Western blot analysis of MEIS1 on different lysates with Rabbit anti-MEIS1 antibody (ET1704-84) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: Mouse brain tissue lysate (20 µg/Lane) Lane 3: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 43 kDa Observed band size: 51 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-84) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |