Anti-MEIS1 antibody [JA13-26]
cat.: ET1704-84
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IP, FC
Clonality: Monoclonal
Clone number: JA13-26
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 47 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human MEIS1 aa 320-370.
Positive control: Human skeletal muscle tissue lysate, mouse brain tissue lysate, K562.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  FC

1:500-1:2,000
1:50-1:100
Uniprot #: SwissProt: O00470 Human | Q60954 Mouse
Alternative names: Homeo box protein Meis1 antibody Homeobox protein Meis1 antibody Leukemogenic homolog protein antibody MEIS 1 antibody Meis homeo box 1 antibody Meis homeobox 1 antibody Meis1 antibody Meis1 mouse homolog antibody Meis1 myeloid ecotropic viral integration site 1 homolog antibody Meis1 myeloid ecotropic viral integration site 1 homolog mouse antibody MEIS1 protein antibody MEIS1_HUMAN antibody MGC43380 antibody Myeloid ecotropic viral integration site 1 homolog antibody WUGSC:H NH0444B04.1 antibody
Images
ET1704-84_1.jpg Fig1: Western blot analysis of MEIS1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-84, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Human skeletal muscle tissue lysate
Lane 2: Mouse brain tissue lysate
ET1704-84_2.jpg Fig2: Flow cytometric analysis of MEIS1 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1704-84, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.