MEIS1 Recombinant Rabbit Monoclonal Antibody [JA13-26]
cat.: ET1704-84
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IP
Clonality: Monoclonal
Clone number: JA13-26
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 43 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human MEIS1 aa 328-377 / 390.
Positive control: HeLa cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB

1:500-1:2,000
Uniprot #: SwissProt: O00470 Human | Q60954 Mouse
Entrez Gene: 686117 Rat
Alternative names: Homeo box protein Meis1 Homeobox protein Meis1 Leukemogenic homolog protein MEIS 1 Meis homeo box 1 Meis homeobox 1 Meis1 Meis1 mouse homolog Meis1 myeloid ecotropic viral integration site 1 homolog Meis1 myeloid ecotropic viral integration site 1 homolog mouse MEIS1 protein MEIS1_HUMAN MGC43380 Myeloid ecotropic viral integration site 1 homolog WUGSC:H NH0444B04.1
Images
ET1704-84_1.jpg Fig1: Western blot analysis of MEIS1 on different lysates with Rabbit anti-MEIS1 antibody (ET1704-84) at 1/2,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: Mouse brain tissue lysate (20 µg/Lane)
Lane 3: Rat brain tissue lysate (40 µg/Lane)

Predicted band size: 43 kDa
Observed band size: 51 kDa

Exposure time: 10 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-84) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.