RBP4 Recombinant Rabbit Monoclonal Antibody [JA33-10]
cat.: ET1704-86
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JA33-10
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 23 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human RBP4 aa152-201 / 201.
Positive control: HepG2 cell lysates, human serum, human liver carcinoma tissue, human kidney tissue, human liver tissue.
Subcellular location: Secreted.
Recommended Dilutions:
  WB
  IHC-P
1:5,000
1:50-1:200
Uniprot #: SwissProt: P02753 Human
Alternative names: OTTHUMP00000020114 OTTHUMP00000020115 OTTHUMP00000020116 Plasma retinol binding protein 4 Plasma retinol-binding protein Plasma retinol-binding protein(1-176) PRBP PRO2222 RBP RBP4 RDCCAS RET4_HUMAN Retinol binding protein 4 Retinol binding protein 4 interstitial Retinol binding protein 4 plasma
Images
ET1704-86_1.jpg Fig1: Western blot analysis of RBP4 on HepG2 cell lysates with Rabbit anti-RBP4 antibody (ET1704-86) at 1/5,000 dilution.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 23 kDa
Observed band size: 21 kDa

Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-86) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1704-86_2.jpg Fig2: Western blot analysis of RBP4 on human serum. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-86, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET1704-86_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-RBP4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-86, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-86_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-RBP4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-86, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-86_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-RBP4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-86, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.