5HT2C Receptor Recombinant Rabbit Monoclonal Antibody [JA31-31]
cat.: ET1704-87
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JA31-31 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 52 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human 5HT2C Receptor aa 409-458 / 458. |
| Positive control: | K-562 cell lysate, SH-SY5Y cell lysate, human brain tissue lysate, mouse brain tissue, rat brain tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:50 |
| Uniprot #: | SwissProt: P28335 Human | P34968 Mouse | P08909 Rat |
| Alternative names: | 5 Hydroxytryptamine 2C receptor 5-HT-1C 5-ht-1c receptor 5-HT-2C 5-HT1C 5-HT2C 5-HTR2C 5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled 5-hydroxytryptamine receptor 1C 5-hydroxytryptamine receptor 2C 5HT1C 5HT2C 5HT2C_HUMAN 5HTR2C 5Hydroxytryptamine 2C receptor Htr1c HTR2C serotonin 1c receptor serotonin 2c receptor Serotonin 5-HT-2C receptor Serotonin receptor 2C |
Images
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Fig1:
Western blot analysis of 5HT2C Receptor on different lysates with Rabbit anti-5HT2C Receptor antibody (ET1704-87) at 1/1,000 dilution. Lane 1: K-562 cell lysate (15 µg/Lane) Lane 2: SH-SY5Y cell lysate (15 µg/Lane) Lane 3: Human brain tissue lysate (30 µg/Lane) Predicted band size: 52 kDa Observed band size: 70 kDa Exposure time: 1 minute 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-87) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-5HT2C Receptor antibody (ET1704-87) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-87) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-5HT2C Receptor antibody (ET1704-87) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-87) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.