Calpain 2 Recombinant Rabbit Monoclonal Antibody [JA43-41]
cat.: ET1704-90
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat, Mouse |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JA43-41 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 80 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Calpain 2 aa 651-700 / 700. |
| Positive control: | Hela cell lysate, rat skin tissue lysate, HepG2, human lung tissue, human kidney tissue, Hela. |
| Subcellular location: | Cytoplasm. Cell membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:2,000 1:50 1:50-1:200 1:50 |
| Uniprot #: | SwissProt: P17655 Human | O08529 Mouse | Q07009 Rat |
| Alternative names: | Calcium activated neutral proteinase 2 Calcium activated neutral proteinase Calcium-activated neutral protease 2, catalytic subunit Calcium-activated neutral proteinase 2 CALP80 Calpain 2 (m/II) large subunit Calpain 2 catalytic subunit Calpain 2 large catalytic subunit Calpain 2 large subunit Calpain 2, large [catalytic] subunit Calpain large polypeptide L2 Calpain M type Calpain M-type Calpain-2 catalytic subunit Calpain-2 large subunit Calpain2 CAN2_HUMAN CANP 2 CANP L2 CANP2 CANPL 2 CANPL2 CANPml Capa2 CAPN 2 CAPN2 FLJ39928 M calpain M calpin M type M-calpain mCANP Millimolar calpain Millimolar-calpain |
Images
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Fig1:
Western blot analysis of Calpain 2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-90, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: Rat skin tissue lysate |
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Fig2: ICC staining of Calpain 2 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-90, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Calpain 2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-90, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Calpain 2 antibody (ET1704-90) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-90) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Flow cytometric analysis of Calpain 2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1704-90, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.