mSin3A Recombinant Rabbit Monoclonal Antibody [JA94-31]
cat.: ET1704-91
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JA94-31 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 145 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human mSin3A aa 1224-1273 / 1273. |
| Positive control: | 293T cell lysates, Hela, MCF-7, NIH/3T3, human kidney tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P FC |
1:500-1:2,000 1:50-1:200 1:50-1:200 1:50 1:50-1:100 |
| Uniprot #: | SwissProt: Q96ST3 Human | Q60520 Mouse Entrez Gene: 363067 Rat |
| Alternative names: | AW553200 DKFZP434K2235 FLJ90319 Histone deacetylase complex subunit Sin 3a Histone deacetylase complex subunit Sin3a KIAA0700 Kiaa4126 mKIAA4126 Paired amphipathic helix protein Sin 3a Paired amphipathic helix protein Sin3a Sin 3a SIN3 homolog A SIN3 homolog A transcription regulator (yeast) SIN3 homolog A transcription regulator SIN3 transcription regulator homolog A Sin3a SIN3A protein SIN3A_HUMAN Transcriptional co repressor Sin 3A Transcriptional co repressor Sin3A Transcriptional corepressor Sin3a Transcriptional regulator SIN3A |
Images
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Fig1: Western blot analysis of mSin3A on 293T cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-91, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining of mSin3A in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-91, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: ICC staining of mSin3A in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-91, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4: ICC staining of mSin3A in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-91, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-mSin3A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-91, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6: Flow cytometric analysis of mSin3A was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1704-91, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.