Peroxiredoxin 3 Recombinant Rabbit Monoclonal Antibody [JA53-21]
cat.: ET1704-92
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IF-Cell, IHC-P, FC, IP
Clonality: Monoclonal
Clone number: JA53-21
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 28 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Peroxiredoxin 3 aa 207-256 / 256.
Positive control: MCF-7 cell lysate, A431 cell lysate, Hela, HepG2, MCF-7, human liver tissue, human liver carcinoma tissue, human kidney tissue.
Subcellular location: Mitochondrion.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
  IP
1:500-1:2,000
1:50-1:200
1:50-1:200
1:50-1:100
Use at an assay dependent concentration.
Uniprot #: SwissProt: P30048 Human
Alternative names: Antioxidant protein 1 AOP 1 AOP-1 AOP1 HBC189 MER5 MGC104387 MGC24293 mitochondrial peroxiredoxin 3 Peroxiredoxin III Peroxiredoxin-3 PRDX3 PRDX3_HUMAN PRO1748 Protein MER5 homolog PRX III Prx-III PRX3 SP 22 SP-22 SP22 Thioredoxin dependent peroxide reductase mitochondrial Thioredoxin-dependent peroxide reductase
Images
ET1704-92_1.jpg Fig1: Western blot analysis of Peroxiredoxin 3 on different lysates with Rabbit anti-Peroxiredoxin 3 antibody (ET1704-92) at 1/500 dilution.

Lane 1: MCF-7 cell lysate
Lane 2: A431 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 28 kDa
Observed band size: 20 kDa

Exposure time: 30 seconds;

15% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-92) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ET1704-92_2.jpg Fig2: ICC staining of Peroxiredoxin 3 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-92, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1704-92_3.jpg Fig3: ICC staining of Peroxiredoxin 3 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-92, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1704-92_4.jpg Fig4: ICC staining of Peroxiredoxin 3 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-92, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1704-92_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Peroxiredoxin 3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-92, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-92_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-Peroxiredoxin 3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-92, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-92_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Peroxiredoxin 3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-92, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-92_8.jpg Fig8: Flow cytometric analysis of Peroxiredoxin 3 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1704-92, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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