Anti-Peroxiredoxin 3 antibody [JA53-21]
cat.: ET1704-92
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, ICC, IHC-P, IP, FC
Clonality: Monoclonal
Clone number: JA53-21
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A purified.
Molecular weight: 24 kDa
Isotype: IgG
Immunogen: recombinant protein
Positive control: Human liver tissue lysate, MCF-7, A431, Hela, HepG2, human liver cancer, human kidney.
Subcellular location: Mitochondrion.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:500-1:2,000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: P30048(Human)
Alternative names: Antioxidant protein 1 antibody AOP 1 antibody AOP-1 antibody AOP1 antibody HBC189 antibody MER5 antibody MGC104387 antibody MGC24293 antibody mitochondrial antibody peroxiredoxin 3 antibody Peroxiredoxin III antibody Peroxiredoxin-3 antibody PRDX3 antibody PRDX3_HUMAN antibody PRO1748 antibody Protein MER5 homolog antibody PRX III antibody Prx-III antibody PRX3 antibody SP 22 antibody SP-22 antibody SP22 antibody Thioredoxin dependent peroxide reductase mitochondrial antibody Thioredoxin-dependent peroxide reductase antibody
Images
ET1704-92_1.jpg Fig1: Western blot analysis of Peroxiredoxin 3 on different cell lysate using anti-Peroxiredoxin 3 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Human liver
Lane 2: MCF-7
Lane 3: A431
ET1704-92_2.jpg Fig2: ICC staining Peroxiredoxin 3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1704-92_3.jpg Fig3: ICC staining Peroxiredoxin 3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1704-92_4.jpg Fig4: ICC staining Peroxiredoxin 3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1704-92_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti- Peroxiredoxin 3 antibody. Counter stained with hematoxylin.
ET1704-92_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti- Peroxiredoxin 3 antibody. Counter stained with hematoxylin.
ET1704-92_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti- Peroxiredoxin 3 antibody. Counter stained with hematoxylin.
ET1704-92_8.jpg Fig8: Flow cytometric analysis of MCF-7 cells with Peroxiredoxin 3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.