IDH2 Recombinant Rabbit Monoclonal Antibody [JA55-31]
cat.: ET1704-93
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JA55-31 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 51 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human IDH2 aa 403-452 / 452. |
| Positive control: | SK-Br-3 cell lysate, THP-1 cell lysate, Jurkat cell lysate, mouse heart tissue lysate, human liver tissue, human tonsil tissue, human prostate tissue, rat heart tissue, mouse heart tissue, MCF-7. |
| Subcellular location: | Mitochondrion. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:2,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P48735 Human | P54071 Mouse | P56574 Rat |
| Alternative names: | D2HGA2 ICD-M IDH IDH2 IDHM IDHP_HUMAN IDP IDPM Isocitrate dehydrogenase [NADP], mitochondrial Isocitrate dehydrogenase 2 (NADP+), mitochondrial mNADP-IDH NADP(+)-specific ICDH Oxalosuccinate decarboxylase |
Images
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Fig1:
Western blot analysis of IDH2 on different lysates with Rabbit anti-IDH2 antibody (ET1704-93) at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate (10 µg/Lane) Lane 2: THP-1 cell lysate (10 µg/Lane) Lane 3: Jurkat cell lysate (10 µg/Lane) Lane 4: Mouse heart tissue lysate (20 µg/Lane) Predicted band size: 51 kDa Observed band size: 45 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-93) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-IDH2 antibody. Counter stained with hematoxylin. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IDH2 antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-IDH2 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-IDH2 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-IDH2 antibody. Counter stained with hematoxylin. |
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Fig7: Flow cytometric analysis of MCF-7 cells with IDH2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.