SNAP23 Recombinant Rabbit Monoclonal Antibody [JA73-15]
cat.: ET1704-95
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JA73-15 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 23 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human SNAP23 aa 162-211 / 211. |
| Positive control: | HeLa cell lysate, HepG2 cell lysate, HEK-293 cell lysate, PC-12 cell lysate, mouse pancreas tissue lysate, human tonsil tissue. |
| Subcellular location: | Cell membrane. Cell junction. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: O00161 Human | O09044 Mouse | O70377 Rat |
| Alternative names: | HsT17016 LS-B8340 SNAP 23 SNAP-23 SNAP23 SNAP23A SNAP23B SNP23_HUMAN Synaptosomal associated protein 23 Synaptosomal associated protein 23kDa Synaptosomal associated protein Synaptosomal-associated protein 23 Vesicle membrane fusion protein SNAP 23 Vesicle membrane fusion protein SNAP23 Vesicle-membrane fusion protein SNAP-23 |
Images
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Fig1:
Western blot analysis of SNAP23 on different lysates with Rabbit anti-SNAP23 antibody (ET1704-95) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: HEK-293 cell lysate (20 µg/Lane) Lane 4: PC-12 cell lysate (20 µg/Lane) Lane 5: Mouse pancreas tissue lysate (40 µg/Lane) Predicted band size: 23 kDa Observed band size: 25 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-95) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SNAP23 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-95, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.