SNAP23 Recombinant Rabbit Monoclonal Antibody [JA73-15]
cat.: ET1704-95
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JA73-15 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 23 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human SNAP23 aa 162-211 / 211. |
| Positive control: | Hela cell lysates, human tonsil tissue, Hela. |
| Subcellular location: | Cell membrane. Cell junction. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:2,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: O00161 Human | O09044 Mouse | O70377 Rat |
| Alternative names: | HsT17016 LS-B8340 SNAP 23 SNAP-23 SNAP23 SNAP23A SNAP23B SNP23_HUMAN Synaptosomal associated protein 23 Synaptosomal associated protein 23kDa Synaptosomal associated protein Synaptosomal-associated protein 23 Vesicle membrane fusion protein SNAP 23 Vesicle membrane fusion protein SNAP23 Vesicle-membrane fusion protein SNAP-23 |
Images
|
Fig1: Western blot analysis of SNAP23 on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-95, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SNAP23 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-95, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3: Flow cytometric analysis of SNAP23 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1704-95, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.