CXCR3 Recombinant Rabbit Monoclonal Antibody [JA61-33]
cat.: ET1704-97
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JA61-33 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 41 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CXCR3 aa 278-298 (Extracellular)/368. |
| Positive control: | Human lung tissue lysate, K-562 cell lysate, HT-29 cell lysate. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB |
1:1,000-1:5,000 |
| Uniprot #: | SwissProt: P49682 Human |
| Alternative names: | C-X-C chemokine receptor type 3 CD 183 CD182 CD183 Chemokine (C X C motif) receptor 3 Chemokine (C X C) receptor 3 Chemokine CXC Motif Receptor 3 CKR L2 CKR-L2 CKRL2 CMKAR3 CXC-R3 CXCR-3 CXCR3 CXCR3_HUMAN G Protein Coupled Receptor 9 G protein-coupled receptor 9 GPR9 Interferon-inducible protein 10 receptor IP-10 receptor IP10 IP10 R IP10 receptor IP10-R IP10R Mig R Mig receptor Mig-R MIGR |
Images
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Fig1:
Western blot analysis of CXCR3 on different lysates with Rabbit anti-CXCR3 antibody (ET1704-97) at 1/1,000 dilution. Lane 1: Human lung tissue lysate (20 µg/Lane) Lane 2: K-562 cell lysate (10 µg/Lane) Predicted band size: 41 kDa Observed band size: 50 kDa Exposure time: 57 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-97) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of CXCR3 on different lysates with Rabbit anti-CXCR3 antibody (ET1704-97) at 1/5,000 dilution. Lane 1: K-562 cell lysate Lane 2: HT-29 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 41 kDa Observed band size: 50 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-97) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.