CXCR3 Recombinant Rabbit Monoclonal Antibody [JA61-33]
cat.: ET1704-97
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IF-Tissue, IP |
| Clonality: | Monoclonal |
| Clone number: | JA61-33 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 41 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CXCR3 aa 278-298 (Extracellular)/368. |
| Positive control: | K562 cell lysate, Hela cell lysate, A431, HepG2, HUVEC.. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue |
1:500-1:2,000 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P49682 Human |
| Alternative names: | C-X-C chemokine receptor type 3 CD 183 CD182 CD183 Chemokine (C X C motif) receptor 3 Chemokine (C X C) receptor 3 Chemokine CXC Motif Receptor 3 CKR L2 CKR-L2 CKRL2 CMKAR3 CXC-R3 CXCR-3 CXCR3 CXCR3_HUMAN G Protein Coupled Receptor 9 G protein-coupled receptor 9 GPR9 Interferon-inducible protein 10 receptor IP-10 receptor IP10 IP10 R IP10 receptor IP10-R IP10R Mig R Mig receptor Mig-R MIGR |
Images
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Fig1:
Western blot analysis of CXCR3 on different lysates with Rabbit anti-CXCR3 antibody (ET1704-97) at 1/500 dilution. Lane 1: K562 cell lysate Lane 2: Hela cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 41 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-97) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining CXCR3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining CXCR3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining CXCR3 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.