CXCR3 Recombinant Rabbit Monoclonal Antibody [JA61-33]
cat.: ET1704-97
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: JA61-33
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 41 kDa
Isotype: IgG
Immunogen: Recombinant protein within human CXCR3 aa 278-298 (Extracellular)/368.
Positive control: Human lung tissue lysate, K-562 cell lysate.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: P49682 Human
Alternative names: C-X-C chemokine receptor type 3 CD 183 CD182 CD183 Chemokine (C X C motif) receptor 3 Chemokine (C X C) receptor 3 Chemokine CXC Motif Receptor 3 CKR L2 CKR-L2 CKRL2 CMKAR3 CXC-R3 CXCR-3 CXCR3 CXCR3_HUMAN G Protein Coupled Receptor 9 G protein-coupled receptor 9 GPR9 Interferon-inducible protein 10 receptor IP-10 receptor IP10 IP10 R IP10 receptor IP10-R IP10R Mig R Mig receptor Mig-R MIGR
Images
ET1704-97_1.jpg Fig1: Western blot analysis of CXCR3 on different lysates with Rabbit anti-CXCR3 antibody (ET1704-97) at 1/1,000 dilution.

Lane 1: Human lung tissue lysate (20 µg/Lane)
Lane 2: K-562 cell lysate (10 µg/Lane)

Predicted band size: 41 kDa
Observed band size: 50 kDa

Exposure time: 57 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-97) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.