JAK3 Recombinant Rabbit Monoclonal Antibody [JM66-31]
cat.: ET1705-1
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P, ICC
Clonality: Monoclonal
Clone number: JM66-31
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 125 kDa
Isotype: IgG
Immunogen: Synthetic peptide within N-terminal human JAK3.
Positive control: Hela, human spleen tissue, human tonsil tissue.
Subcellular location: Endomembrane system, Cytoplasm.
Recommended Dilutions:
  WB
  IHC-P
  ICC

1:500
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P52333 Human
Alternative names: EC 2.7.10.2 JAK 3 JAK L JAK-3 Jak3 JAK3 HUMAN JAK3_HUMAN JAKL Janus kinase 3 (a protein tyrosine kinase, leukocyte) Janus kinase 3 Janus Kinase3 L JAK L-JAK Leukocyte janus kinase LJAK Protein tyrosine kinase leukocyte Tyrosine protein kinase JAK3 Tyrosine-protein kinase JAK3
Images
ET1705-1_1.jpg Fig1: ICC staining of JAK3 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1705-1, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1705-1_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-JAK3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-1, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-1_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-JAK3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-1, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.