JAK3 Recombinant Rabbit Monoclonal Antibody [JM66-31]
cat.: ET1705-1
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | JM66-31 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 125 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human JAK3 aa 451-950. |
| Positive control: | TF-1 (Human erythroleukemia cell) cell lysate, Jurkat (Human T-lymphoblastic cells) cell lysate, NK-92 (Human natural killer cell) cell lysate, CTLL-2 (Mouse T lymphocyte) cell lysate, Mouse spleen tissue lysate. |
| Subcellular location: | Endomembrane system, Cytoplasm. |
| Recommended Dilutions:
WB IP |
1:2,000 1-2μg/sample |
| Uniprot #: | SwissProt: P52333 Human | Q62137 Mouse |
| Alternative names: | EC 2.7.10.2 JAK 3 JAK L JAK-3 Jak3 JAK3 HUMAN JAK3_HUMAN JAKL Janus kinase 3 (a protein tyrosine kinase, leukocyte) Janus kinase 3 Janus Kinase3 L JAK L-JAK Leukocyte janus kinase LJAK Protein tyrosine kinase leukocyte Tyrosine protein kinase JAK3 Tyrosine-protein kinase JAK3 |
Images
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Fig1:
Western blot analysis of JAK3 on different lysates with Rabbit anti-JAK3 antibody (ET1705-1) at 1/2,000 dilution. Lane 1: TF-1 (Human erythroleukemia cell) cell lysate (20 µg/Lane) Lane 2: Jurkat (Human T-lymphoblastic cells) cell lysate (20 µg/Lane) Lane 3: NK-92 (Human natural killer cell) cell lysate (20 µg/Lane) Lane 4: HaCaT (Human immortalized keratinocyte) cell lysate (20 µg/Lane) Lane 5: CTLL-2 (Mouse T lymphocyte) cell lysate (20 µg/Lane) Lane 6: Mouse spleen tissue lysate (40 µg/Lane) Exposure time: 1 minute; ECL: K1801 HaCaT is a negative control (PMID: 11709700). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1705-1, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 125 kDa Observed band size: 125 kDa |
|
Fig2:
Immunoprecipitation (IP) JAK3 was immunoprecipitated in 0.2 mg TF-1 (Human erythroleukemia cell) cell lysate with ET1705-1 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1705-1 at 1/1,000 dilution. Anti-Rabbit IgG for IP, AlpSdAbs® VHH(HRP) (025-100-005) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: TF-1 cell lysate (input) Lane 2: ET1705-1 IP in TF-1 cell lysate Lane 3: Rabbit IgG instead of ET1705-1 in TF-1 cell lysate Exposure time: 1 minute Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary dilution: ET1705-1, 1/1,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature Predicted band size: 125 kDa Observed band size: 125 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.