C4 Recombinant Rabbit Monoclonal Antibody [JM88-13]
cat.: ET1705-10
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JM88-13 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 193 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human C4 aa 1,231-1,280 / 1,744. |
| Positive control: | Human liver tissue lysates, HepG2, human liver tissue, human liver carcinoma tissue. |
| Subcellular location: | Cell junction, Cell projection, Secreted, Synapse. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P |
1:500-1:1,000 1:50-1:200 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P0C0L4 Human |
| Alternative names: | Acidic C4 Acidic complement C4 Basic C4 Basic complement C4 C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2 C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3 C4, Chido form C4, Rodgers from C4A anaphylatoxin C4A C4A2 C4A3 C4A4 C4A6 C4AD C4B C4B_2 C4B1 C4B12 C4B2 C4B3 C4BD C4F C4S CH Chido form of C4 CO4 CO4A_HUMAN Complement C4 gamma chain complement C4-A complement C4-B complement C4-B-like complement C4B1a Complement component 4A (Rodgers blood group) Complement component 4A complement component 4B (Chido blood group), copy 2 Complement component 4B (Childo blood group) Complement component 4B Complement component 4F Complement component 4S CPAMD2 CPAMD3 RG Rodgers form of C4 |
Images
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Fig1: Western blot analysis of C4 on human liver tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-10, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining of C4 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1705-10, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-C4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-10, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-C4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-10, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.