DNA Polymerase beta Recombinant Rabbit Monoclonal Antibody [JM93-12]
cat.: ET1705-12
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat, Zebrafish
Applications: WB, IHC-P, IF-Cell, IP
Clonality: Monoclonal
Clone number: JM93-12
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 38 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human DNA Polymerase beta aa 286-335 / 335.
Positive control: HeLa (Human cervical adenocarcinoma cell) cell lysate, SH-SY5Y (Human neuroblastoma cell) cell lysate, HT-29 (Human colorectal adenocarcinoma cell) cell lysate, Neuro-2a (Mouse brain neuroblastoma cell) cell lysate, C6 (Rat glioma cell) cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, K-562, rat lung tissue, human breast carcinoma tissue, human stomach carcinoma tissue, human uterus tissue, mouse cerebellum tissue.
Subcellular location: Nucleus, Cytoplasm.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
  IP
1:5,000
1:50-1:200
1:20-1:50
1:10-1:20
Uniprot #: SwissProt: P06746 Human | Q8K409 Mouse | P06766 Rat
Alternative names: DNA directed DNA polymerase beta DNA pol beta DNA polymerase beta DNA polymerase beta subunit DPOLB_HUMAN MGC125976 Pol B Pol beta POLB Polymerase (DNA directed) beta
Images
ET1705-12_1.jpg Fig1: Western blot analysis of DNA Polymerase beta on different lysates with Rabbit anti-DNA Polymerase beta antibody (ET1705-12) at 1/5,000 dilution.

Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 2: SH-SY5Y (Human neuroblastoma cell) cell lysate
Lane 3: HT-29 (Human colorectal adenocarcinoma cell) cell lysate
Lane 4: Neuro-2a (Mouse brain neuroblastoma cell) cell lysate
Lane 5: C6 (Rat glioma cell) cell lysate
Lane 6: Mouse brain tissue lysate
Lane 7: Rat brain tissue lysate

Cell Lysates at 20 µg/Lane, Tissue Lysates at 40 µg/Lane.
Exposure time: 25 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET1705-12, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 38 kDa
Observed band size: 38 kDa
ET1705-12_2.jpg Fig2: Immunocytochemistry analysis of K-562 cells labeling DNA Polymerase beta with Rabbit anti-DNA Polymerase beta antibody (ET1705-12) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-DNA Polymerase beta antibody (ET1705-12) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
ET1705-12_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-DNA Polymerase beta antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-12, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-12_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-DNA Polymerase beta antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-12, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-12_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-DNA Polymerase beta antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-12, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-12_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-DNA Polymerase beta antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-12, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-12_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-DNA Polymerase beta antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-12, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.