MUC4 Recombinant Rabbit Monoclonal Antibody [JM19-36]
cat.: ET1705-13
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JM19-36 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 542 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human MUC4 aa 4,688-5,412 / 5,412. |
| Positive control: | Capan-1 (Human pancreatic cancer cell) cell lysate, SW 1990 (Human pancreatic cancer cell) cell lysates. |
| Subcellular location: | Secreted, Cell membrane and Membrane. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:100-1:1,000 |
| Uniprot #: | SwissProt: Q99102 Human |
| Alternative names: | Ascites sialoglycoprotein 1 Ascites sialoglycoprotein 2 Ascites sialoglycoprotein ASGP ASGP-1 ASGP-2 HSA276359 MUC 4 MUC-4 Muc4 MUC4_HUMAN Mucin 4 Mucin 4 cell surface associated Mucin 4 tracheobronchial Mucin-4 beta chain Pancreatic adenocarcinoma mucin Testis mucin Tracheobronchial mucin Tracheobronchial mucin Fragment |
Images
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Fig1:
Western blot analysis of MUC4 on different lysates with Rabbit anti-MUC4 antibody (ET1705-13) at 1/5,000 dilution. Lane 1: PANC-1 (Human epidermoid carcinoma pancreatic ductal cell) cell lysate Lane 2: Capan-1 (Human pancreatic cancer cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 42 seconds; ECL: K1801 PANC-1 is a negative control (PMID : 23152882). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1705-13, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 542 kDa Observed band size: 100-170 kDa |
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Fig2:
Western blot analysis of MUC4 on SW 1990 (Human pancreatic cancer cell) cell lysate with Rabbit anti-MUC4 antibody (ET1705-13) at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1705-13, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 542 kDa Observed band size: 100-170 kDa |
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Fig3:
Application: Immunohistochemistry (IHC-P) Species: Human Tissue: Tonsil Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: ET1705-13, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig4:
Application: Immunohistochemistry (IHC-P) Species: Human Tissue: Ovarian serous carcinoma Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: ET1705-13, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig5:
Application: Immunohistochemistry (IHC-P) Species: Human Tissue: Colon Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: ET1705-13, 1/100, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig6:
Application: Immunohistochemistry (IHC-P) Species: Human Tissue: Pancreas Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: ET1705-13, 1/100, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. Negative expression of MUC4 protein in pancreas is consistent with the predicted expression pattern. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.