TAK1 Recombinant Rabbit Monoclonal Antibody [JM73-19]
cat.: ET1705-14
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JM73-19 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 67 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal human TAK1. |
| Positive control: | A431 cell lysate, A549 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, Mouse lung tissue lysate, Rat lung tissue lysate, HeLa cell lysate, COS-1 cell lysate. |
| Subcellular location: | Cytoplasm, Cell membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: O43318 Human | Q62073 Mouse | P0C8E4 Rat |
| Alternative names: | M3K7_HUMAN MAP3K 7 Map3k7 MEKK7 Mitogen activated protein kinase kinase kinase 7 Mitogen-activated protein kinase kinase kinase 7 TAK1 TGF beta activated kinase 1 TGF-beta-activated kinase 1 TGF1a Transforming growth factor beta activated kinase 1 Transforming growth factor-beta-activated kinase 1 |
Images
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Fig1:
Western blot analysis of TAK1 on different lysates with Rabbit anti-TAK1 antibody (ET1705-14) at 1/1,000 dilution. Lane 1: A549-WT cell lysate Lane 2: A549-KD TAK1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 67 kDa Observed band size: 72 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-14) at 1/1,000 dilution was used in 2% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of TAK1 on different lysates with Rabbit anti-TAK1 antibody (ET1705-14) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 µg/Lane) Lane 2: A549 cell lysate (20 µg/Lane) Lane 3: NIH/3T3 cell lysate (20 µg/Lane) Lane 4: C6 cell lysate (20 µg/Lane) Lane 5: Mouse lung tissue lysate (40 µg/Lane) Lane 6: Rat lung tissue lysate (40 µg/Lane) Predicted band size: 67 kDa Observed band size: 72 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-14) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Western blot analysis of TAK1 on different lysates with Rabbit anti-TAK1 antibody (ET1705-14) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 67 kDa Observed band size: 67 kDa Exposure time: 15 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-14) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.