Anti-Fascin antibody [JM12-53]
cat.: ET1705-18
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC-P, IP, FC
Clonality: Monoclonal
Clone number: JM12-53
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 55 kDa
Isotype: IgG
Immunogen: Recombinant protein within human Fascin aa 230-380.
Positive control: Mouse testis tissue lysate, mouse spleen tissue lysate, SH-SY5Y cell lysate, human brain tissue lysate, Hela, SW480, SH-SY5Y, rat brain tissue, human kidney tissue, human tonsil tissue.
Subcellular location: Cytoskeleton, stress fiber, cytosol, cell cortex, filopodium, invadopodium, microvillus, cell junction.
Recommended Dilutions:
  WB
  ICC/IF
  IHC-P
  FC
  IP

1:500-1:2,000
1:50-1:200
1:50-1:200
1:50-1:100
Use at an assay dependent concentration.
Uniprot #: SwissProt: Q16658 Human | Q61553 Mouse | P85845 Rat
Alternative names: 55 kDa actin bundling protein antibody 55 kDa actin-bundling protein antibody Actin bundling protein antibody actin bundling protein, 55-KD antibody FAN 1 antibody FAN1 antibody Fascin 1 antibody Fascin actin bundling protein 1 antibody Fascin antibody Fascin homolog 1 actin bundling protein (Strongylocentrotus purpuratus) antibody Fascin homolog 1 antibody Fascin, sea urchin, homolog of, 1 antibody Fascin1 antibody FLJ38511 antibody FSCN 1 antibody FSCN1 antibody FSCN1_HUMAN antibody HSN antibody p55 antibody Singed (Drosophila) like (sea urchin fascin homolog like) antibody Singed drosophila homolog like antibody Singed like (fascin homolog sea urchin) (Drosophila) antibody Singed like (fascin homolog sea urchin) antibody Singed like protein antibody Singed, drosophila, homolog of antibody Singed-like protein antibody SNL antibody Strongylocentrotus purpuratus antibody
Images
ET1705-18_1.jpg Fig1: Western blot analysis of Fascin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-18, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: mouse testis tissue lysate
Lane 2: mouse spleen tissue lysate
Lane 3: SH-SY5Y cell lysate
Lane 4: human brain tissue lysate
ET1705-18_2.jpg Fig2: ICC staining of Fascin in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1705-18, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1705-18_3.jpg Fig3: ICC staining of Fascin in SW480 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1705-18, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1705-18_4.jpg Fig4: ICC staining of Fascin in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1705-18, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1705-18_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Fascin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-18, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-18_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Fascin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-18, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-18_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Fascin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-18, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-18_8.jpg Fig8: Flow cytometric analysis of Fascin was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1705-18, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.