Calretinin Recombinant Rabbit Monoclonal Antibody [JM12-93]
cat.: ET1705-19
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IHC-Fr, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JM12-93 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 32 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Calretinin aa 60-100. |
| Positive control: | Mouse brain tissue lysate, rat brain tissue lysate, mouse hippocampus tissue lysate, rat hippocampus tissue lysate, human brain tissue, mouse brain tissue, rat brain tissue, mouse hippocampus tissue, mouse cerebral cortex tissue. |
| Subcellular location: | Cytosol, dendrite, gap junction, nucleus, parallel fiber to Purkinje cell synapse, synapse, terminal bouton. |
| Recommended Dilutions:
WB IHC-P IHC-Fr IF-Tissue |
1:2,000 1:5,000 1:100 1:1,000 |
| Uniprot #: | SwissProt: P22676 Human | Q08331 Mouse | P47728 Rat |
| Alternative names: | 29 kDa calbindin CAB 29 CAB29 CAL 2 CAL2 CALB 2 CALB2 CALB2_HUMAN Calbindin 2 29kDa Calbindin 2 Calbindin D29K Calbindin2 Calretinin CR |
Images
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Fig1:
Western blot analysis of Calretinin on different lysates with Rabbit anti-Calretinin antibody (ET1705-19) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse hippocampus tissue lysate Lane 4: Rat hippocampus tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 32 kDa Observed band size: 27 kDa Exposure time: 7 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-19) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Calretinin antibody (ET1705-19) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-19) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Calretinin antibody (ET1705-19) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-19) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Calretinin antibody (ET1705-19) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-19) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunofluorescence analysis of frozen mouse hippocampus tissue labeling Calretinin with Rabbit anti-Calretinin antibody (ET1705-19). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody ((ET1705-19, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner. |
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Fig6:
Immunofluorescence analysis of frozen mouse cerebral cortex tissue labeling Calretinin with Rabbit anti-Calretinin antibody (ET1705-19). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody ((ET1705-19, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.