NDRG1 Recombinant Rabbit Monoclonal Antibody [JM32-02]
cat.: ET1705-20
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P, IP
Clonality: Monoclonal
Clone number: JM32-02
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 43 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human NDRG1 aa 345-394 / 394.
Positive control: PC-3 cell lysate, SW480 cell lysate, RPMI 8226 cell lysate, RAW264.7 cell lysate, Mouse eyeball tissue lysate, Rat eyeball tissue lysate, MCF-7, A431, human prostate tissue, mouse kidney tissue, human colon carcinoma tissue.
Subcellular location: Cytoplasm. Nucleus. Cell membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
  IP
1:500-1:2,000
1:50-1:200
1:50-1:200
1:200-1:1,000
1:50
Uniprot #: SwissProt: Q92597 Human | Q62433 Mouse | Q6JE36 Rat
Alternative names: 42 kDa Anti GC4 cap43 cmt4d Differentiation related gene1 protein Differentiation-related gene 1 protein Drg 1 DRG-1 drg1 gc4 GC4 hmsnl Human mRNA for RTP complete cds N myc downstream regulated gene 1 N myc downstream regulated gene 1 protein N-myc downstream-regulated gene 1 protein Ndr 1 ndr1 NDRG 1 Ndrg1 NDRG1 protein NDRG1_HUMAN Nickel specific induction protein Nickel specific induction protein Cap43 Nickel-specific induction protein Cap43 nmsl Nmyc downstream regulated Nmyc downstream regulated gene1 Nmyc downstream regulated gene1 protein Protein NDRG1 Protein regulated by oxygen 1 Protein regulated by oxygen1 Proxy1 Reduced in tumor Reducin Reducing agents and tunicamycin responsive protein Reducing agents and tunicamycin-responsive protein Rit42 RTP targ1 TDD5 tdds Tunicamycin responsive protein
Images
ET1705-20_1.jpg Fig1: Western blot analysis of NDRG1 on different lysates with Rabbit anti-NDRG1 antibody (ET1705-20) at 1/1,000 dilution.

Lane 1: PC-3 cell lysate (20 µg/Lane)
Lane 2: SW480 cell lysate (20 µg/Lane)
Lane 3: RPMI 8226 cell lysate (20 µg/Lane)
Lane 4: RAW264.7 cell lysate (20 µg/Lane)
Lane 5: Mouse eyeball tissue lysate (40 µg/Lane)
Lane 6: Rat eyeball tissue lysate (40 µg/Lane)

Lysates/proteins at 10 µg/Lane.

Predicted band size: 43 kDa
Observed band size: 48 kDa

Exposure time: 6 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-20) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1705-20_2.jpg Fig2: ICC staining of NDRG1 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1705-20, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1705-20_3.jpg Fig3: ICC staining of NDRG1 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1705-20, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1705-20_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human prostate tissue with Rabbit anti-NDRG1 antibody (ET1705-20) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-20) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-20_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-NDRG1 antibody (ET1705-20) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-20) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-20_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-NDRG1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-20, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.