Insulin Receptor R Recombinant Rabbit Monoclonal Antibody [JM22-38]
cat.: ET1705-22
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JM22-38
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 144 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Insulin Receptor R aa 12-61 / 1,297.
Positive control: Human liver carcinoma tissue, human colon carcinoma tissue, human kidney tissue, mouse testis tissue, mouse kidney tissue.
Subcellular location: Membrane.
Recommended Dilutions:
  WB
  IHC-P
1:500
1:50-1:200
Uniprot #: SwissProt: P14616 Human | Q9WTL4 Mouse | Q64716 Rat
Alternative names: INSRR INSRR_HUMAN Insulin receptor related receptor precursor Insulin receptor-related protein alpha chain Insulin receptor-related protein beta chain insulin receptor-related receptor IR R IR related receptor IR-related receptor IRR Sir r Sirr
Images
ET1705-22_1.jpg Fig1: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-Insulin Receptor R antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-22, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-22_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Insulin Receptor R antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-22, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-22_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Insulin Receptor R antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-22, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-22_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Insulin Receptor R antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-22, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-22_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Insulin Receptor R antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-22, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.