ETS1 Recombinant Rabbit Monoclonal Antibody [JM92-32]
cat.: ET1705-23
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: JM92-32
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 50 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human ETS1 aa 10-59 / 441.
Positive control: HeLa cell lysate, Jurkat cell lysate, Daudi cell lysate, human tonsil tissue, human spleen tissue, mouse spleen tissue, Jurkat.
Subcellular location: Nucleus, Cytoplasm.
Recommended Dilutions:
  WB
  IHC-P
  FC
1:5,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P14921 Human | P27577 Mouse
Alternative names: Avian erythroblastosis virus E26 (v ets) oncogene homolog 1 C ets 1 protein c-ets-1 ETS 1 Ets protein ETS proto-oncogene 1, transcription factor ETS1 ETS1 oncogene ETS1 protein ETS1_HUMAN EWSR 2 EWSR2 FLJ10768 Oncogene ETS1 P54 Protein C-ets-1 v ets avian erythroblastosis virus E2 oncogene homolog v ets avian erythroblastosis virus E2 oncogene homolog 1 v ets avian erythroblastosis virus E26 oncogene homolog 1 v ets erythroblastosis virus E26 oncogene homolog 1 v-ets erythroblastosis virus E26 oncogene homolog 1
Images
ET1705-23_1.jpg Fig1: Western blot analysis of ETS1 on different lysates with Rabbit anti-ETS1 antibody (ET1705-23) at 1/5,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: Jurkat cell lysate
Lane 3: Daudi cell lysate

Lysates/proteins at 15 µg/Lane.

Predicted band size: 50 kDa
Observed band size: 50 kDa

Exposure time: 24 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-23) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1705-23_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ETS1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-23, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-23_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-ETS1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-23, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-23_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-ETS1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-23, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-23_5.jpg Fig5: Flow cytometric analysis of ETS1 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1705-23, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.