Thymidylate Synthase Recombinant Rabbit Monoclonal Antibody [JM33-20]
cat.: ET1705-24
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | JM33-20 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 36 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Thymidylate Synthase aa 264-313 / 313. |
| Positive control: | NIH/3T3 cell lysate, RAW264.7 cell lysate, C6 cell lysate, HeLa cell lysate, Mouse thymus tissue lysate, human tonsil tissue, mouse colon tissue, rat bone tissue, Hela. |
| Subcellular location: | Nucleus. Cytoplasm. Mitochondrion. |
| Recommended Dilutions:
WB IHC-P FC IP |
1:500-1:2,000 1:50-1:200 1:50-1:100 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P04818 Human | P07607 Mouse | P45352 Rat |
| Alternative names: | d TMP synthase EC 2.1.1.45 HsT422 MGC88736 OTTHUMP00000162195 Thymidylate synthase Thymidylate synthetase TMS TS TSase Tyms TYMS protein Tyms thymidylate synthetase TYSY_HUMAN |
Images
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Fig1:
Western blot analysis of Thymidylate Synthase on different lysates with Rabbit anti-Thymidylate Synthase antibody (ET1705-24) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate (20 µg/Lane) Lane 2: RAW264.7 cell lysate (20 µg/Lane) Lane 3: C6 cell lysate (20 µg/Lane) Lane 4: HeLa cell lysate (20 µg/Lane) Lane 5: Mouse thymus tissue lysate (40 µg/Lane) Predicted band size: 36 kDa Observed band size: 35 kDa Exposure time: Lane 1-3: 3 minutes; Lane 4-5: 46 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-24) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Thymidylate Synthase antibody (ET1705-24) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-24) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Thymidylate Synthase antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded rat bone tissue using anti-Thymidylate Synthase antibody. Counter stained with hematoxylin. |
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Fig5: Flow cytometric analysis of Hela cells with Thymidylate Synthase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.