KAT7 Recombinant Rabbit Monoclonal Antibody [JM32-39]
cat.: ET1705-25
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JM32-39 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 71 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human KAT7 aa 1-240 / 611. |
| Positive control: | HeLa cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, mouse testis tissue, rat testis tissue, mouse fallopian tubes tissue, HCT 116. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:1,000 1:50-1:100 1:50 |
| Uniprot #: | SwissProt: O95251 Human | Q5SVQ0 Mouse | Q810T5 Rat |
| Alternative names: | Hbo 1 HBO1 HBOa Histone acetyltransferase binding to hORC1 Histone acetyltransferase binding to ORC1 Histone acetyltransferase KAT7 Histone acetyltransferase MYST2 K(lysine) acetyltransferase 7 KAT 7 KAT7 Lysine acetyltransferase 7 MOZ MOZ YBF2/SAS3 SAS2 and TIP60 protein MOZ, YBF2/SAS3, SAS2 and TIP60 protein 2 MYST 2 MYST histone acetyltransferase 2 MYST protein 2 MYST-2 MYST2 MYST2_HUMAN SAS 2 SAS2 and TIP60 protein 2 TIP60 protein 2 YBF2/SAS3 ZC2HC7 |
Images
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Fig1:
Western blot analysis of KAT7 on different lysates with Rabbit anti-KAT7 antibody (ET1705-25) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: NIH/3T3 cell lysate (20 µg/Lane) Lane 3: RAW264.7 cell lysate (20 µg/Lane) Lane 4: Mouse testis tissue lysate (40 µg/Lane) Lane 5: Rat testis tissue lysate (40 µg/Lane) Predicted band size: 71 kDa Observed band size: 75 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-25) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-KAT7 antibody. Counter stained with hematoxylin. |
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Fig3: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-KAT7 antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse fallopian tubes tissue using anti-KAT7 antibody. Counter stained with hematoxylin. |
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Fig5:
Immunocytochemistry analysis of HCT 116 cells labeling KAT7 with Rabbit anti-KAT7 antibody (ET1705-25) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-KAT7 antibody (ET1705-25) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.