Anti-Cytochrome P450 2D6 antibody [JM44-23]
cat.: ET1705-28
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, ICC/IF, IHC-P, FC
Clonality: Monoclonal
Clone number: JM44-23
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A purified.
Molecular weight: 56 kDa
Isotype: IgG
Immunogen: Recombinant protein
Positive control: HepG2, SW480, human liver tissue, human kidney tissue.
Subcellular location: Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Recommended Dilutions:
  WB
  ICC/IF
  IHC-P
  FC

1:500-1:2,000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: P10635(Human)
Alternative names: CPD6 antibody CYP2D antibody CYP2D6 antibody CYP2D7AP antibody CYP2D7BP antibody CYP2D7P2 antibody CYP2D8P2 antibody CYP2DL1 antibody CYPIID6 antibody Cytochrome P450 DB1 antibody Cytochrome P450 family 2 subfamily D member 6 antibody Cytochrome P450 family 2 subfamily D polypeptide 6 antibody Debrisoquine 4 hydroxylase antibody Flavoprotein linked monooxygenase antibody Microsomal monooxygenase antibody P450 DB1 antibody P450C2D antibody P450DB1 antibody Xenobiotic monooxygenase antibody
Images
ET1705-28_1.jpg Fig1: Western blot analysis of Cytochrome P450 2D6 on HepG2 cell using anti-Cytochrome P450 2D6 antibody at 1/1,000 dilution.
ET1705-28_2.jpg Fig2: ICC staining Cytochrome P450 2D6 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1705-28_3.jpg Fig3: ICC staining Cytochrome P450 2D6 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1705-28_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Cytochrome P450 2D6 antibody. Counter stained with hematoxylin.
ET1705-28_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Cytochrome P450 2D6 antibody. Counter stained with hematoxylin.
ET1705-28_6.jpg Fig6: Flow cytometric analysis of SW480 cells with Cytochrome P450 2D6 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.