TGFBI Recombinant Rabbit Monoclonal Antibody [JM24-53]
cat.: ET1705-29
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JM24-53 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 75 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human TGFBI aa 93-142 / 683. |
| Positive control: | Mouse small intestine tissue lysate, Mouse colon tissue lysate, Rat colon tissue lysate, Rat uterus tissue lysate, human colon tissue, human colon cancer tissue. |
| Subcellular location: | Secreted, extracellular space, extracellular matrix. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200 |
| Uniprot #: | SwissProt: Q15582 Human | P82198 Mouse | D4A8G5 Rat |
| Alternative names: | RGD containing collagen associated protein AI181842 AI747162 Beta ig Beta ig h3 Beta ig-h3 BGH3_HUMAN Big h3 BIGH3 CDB1 CDG2 CDGG1 CSD CSD1 CSD2 CSD3 EBMD Kerato epithelin Kerato-epithelin LCD1 MGC150270 RGD CAP RGD-CAP RGD-containing collagen-associated protein TGFBI TGFBI transforming growth factor, beta induced, 68kDa Transforming growth factor beta induced protein ig h3 Transforming growth factor-beta-induced protein ig-h3 |
Images
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Fig1:
Western blot analysis of TGFBI on different lysates with Rabbit anti-TGFBI antibody (ET1705-29) at 1/1,000 dilution. Lane 1: Mouse small intestine tissue lysate (20 µg/Lane) Lane 2: Mouse colon tissue lysate (20 µg/Lane) Lane 3: Rat colon tissue lysate (20 µg/Lane) Lane 4: Rat uterus tissue lysate (20 µg/Lane) Predicted band size: 75 kDa Observed band size: 68 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-29) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-TGFBI antibody (ET1705-29) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-29) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-TGFBI antibody (ET1705-29) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-29) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.