Ephrin B2 Recombinant Rabbit Monoclonal Antibody [JM53-21]
cat.: ET1705-33
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JM53-21 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 37 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Ephrin B aa 47-96 / 333. |
| Positive control: | SH-SY5Y cell lysate, SK-OV-3 cell lysate, mouse lung tissue lysate, rat brain tissue lysate, HeLa, Neuro-2a, C6. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IF-Cell |
1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: P52799 Human | P52800 Mouse | B2B9A9 Rat |
| Alternative names: | EFN B2 EFNB 2 Efnb2 EFNB2_HUMAN Eph related receptor tyrosine kinase ligand 5 EPH-related receptor tyrosine kinase ligand 5 ephrin B2 Ephrin-B2 EphrinB2 EPLG 5 EPLG5 Htk L HTK ligand HTK-L HTKL LERK 5 LERK-5 LERK5 Ligand of eph related kinase 5 MGC126226 MGC126227 MGC126228 OTTMUSP00000024973 |
Images
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Fig1:
Western blot analysis of Ephrin B2 on different lysates with Rabbit anti-Ephrin B2 antibody (ET1705-33) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate (15 µg/Lane) Lane 2: SK-OV-3 cell lysate (15 µg/Lane) Lane 3: Mouse lung tissue lysate (20 µg/Lane) Lane 4: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 37 kDa Observed band size: 50 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-33) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HeLa cells labeling Ephrin B2 with Rabbit anti-Ephrin B2 antibody (ET1705-33) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ephrin B2 antibody (ET1705-33) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunocytochemistry analysis of Neuro-2a cells labeling Ephrin B2 with Rabbit anti-Ephrin B2 antibody (ET1705-33) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ephrin B2 antibody (ET1705-33) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig4:
Immunocytochemistry analysis of C6 cells labeling Ephrin B2 with Rabbit anti-Ephrin B2 antibody (ET1705-33) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ephrin B2 antibody (ET1705-33) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.