ACE Recombinant Rabbit Monoclonal Antibody [JM59-32]
cat.: ET1705-36
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JM59-32 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 150 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human ACE aa 1,257-1,306 / 1,306. |
| Positive control: | Human lung tissue lysate, mouse lung tissue lysate, mouse kidney tissue lysate, human kidney tissue, mouse testis tissue, mouse kidney tissue. |
| Subcellular location: | Secreted. Cell membrane. Cytoplasm. |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000-1:5,000 1:100-1:500 1:50-1:100 |
| Uniprot #: | SwissProt: P12821 Human | P09470 Mouse |
| Alternative names: | ACE 1 ACE ACE T ACE_HUMAN ACE1 Angiotensin converting enzyme somatic isoform Angiotensin converting enzyme testis specific isoform Angiotensin I converting enzyme 1 Angiotensin I converting enzyme Angiotensin I converting enzyme peptidyl dipeptidase A 1 angiotensin I converting enzyme peptidyl-dipeptidase A 1 transcript Angiotensin-converting enzyme Carboxycathepsin CD 143 CD143 CD143 antigen DCP 1 DCP DCP1 Dipeptidyl carboxypeptidase 1 Dipeptidyl carboxypeptidase I Kininase II MGC26566 MVCD3 Peptidase P Peptidyl dipeptidase A soluble form Testicular ECA |
Images
|
Fig1:
Western blot analysis of ACE on different lysates with Rabbit anti-ACE antibody (ET1705-36) at 1/5,000 dilution. Lane 1: Human lung tissue lysate Lane 2: Mouse lung tissue lysate Lane 3: Mouse kidney tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 150 kDa Observed band size: 150-170 kDa Exposure time: 2 minutes 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-36) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ACE antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-36, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ACE antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-36, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ACE antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-36, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Western blot analysis of ACE on rat kidney tissue lysates with Rabbit anti-ACE antibody (ET1705-36) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 150 kDa Observed band size: 170 kDa Exposure time: 1 minutes 150 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-36) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.