PKC mu Recombinant Rabbit Monoclonal Antibody [JM30-17]
cat.: ET1705-4
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Monoclonal
Clone number: JM30-17
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 102 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human PKC aa 201-250 / 912.
Positive control: Hela cell lysate, mouse kidney tissue lysate, PC-12 cell lysate, PC-3 cell lysates.
Subcellular location: Cell membrane, trans-Golgi network, Cytoplasm.
Recommended Dilutions:
  WB

1:500-1:2,000
Uniprot #: SwissProt: Q15139 Human | Q62101 Mouse | Q9WTQ1 Rat
Alternative names: KPCD1_HUMAN nPKC D1 nPKC mu nPKC-D1 nPKC-mu nPKCD1 nPKCmu PKC PKC MU PKCM PKCmu PKD 1 PKD PKD1 PRKCM PRKD 1 Prkd1 Protein kinase C mu Protein kinase C mu type Protein kinase D Protein kinase D1 Serine/threonine protein kinase D1 Serine/threonine-protein kinase D1
Images
ET1705-4_1.jpg Fig1: Western blot analysis of PKC mu on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-4, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: Mouse kidney tissue lysate
Lane 3: PC-12 cell lysate
ET1705-4_2.jpg Fig2: Western blot analysis of PKC mu on PC-3 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-4, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.