PKC mu Recombinant Rabbit Monoclonal Antibody [JM30-17]
cat.: ET1705-4
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JM30-17 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 102 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human PKC aa 201-250 / 912. |
| Positive control: | Hela cell lysate, mouse kidney tissue lysate, PC-12 cell lysate, PC-3 cell lysates. |
| Subcellular location: | Cell membrane, trans-Golgi network, Cytoplasm. |
| Recommended Dilutions:
WB |
1:500-1:2,000 |
| Uniprot #: | SwissProt: Q15139 Human | Q62101 Mouse | Q9WTQ1 Rat |
| Alternative names: | KPCD1_HUMAN nPKC D1 nPKC mu nPKC-D1 nPKC-mu nPKCD1 nPKCmu PKC PKC MU PKCM PKCmu PKD 1 PKD PKD1 PRKCM PRKD 1 Prkd1 Protein kinase C mu Protein kinase C mu type Protein kinase D Protein kinase D1 Serine/threonine protein kinase D1 Serine/threonine-protein kinase D1 |
Images
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Fig1:
Western blot analysis of PKC mu on different lysates with Rabbit anti-PKC mu antibody (ET1705-4) at 1/1,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si PKC mu cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 102 kDa Observed band size: 102 kDa Exposure time: 40 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-4) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of PKC mu on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-4, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: Mouse kidney tissue lysate Lane 3: PC-12 cell lysate |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.