IKB epsilon Recombinant Rabbit Monoclonal Antibody [JM62-63]
cat.: ET1705-40
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JM62-63 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 53 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human IKB epsilon aa 175-224 / 500. |
| Positive control: | THP-1 cell lysate, Jurkat cell lysate, Hela cell lysate, human tonsil tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: O00221 Human |
| Alternative names: | I kappa B epsilon I-kappa-B-epsilon IkappaBepsilon IkB E IkB-E IkB-epsilon IKBE IKBE_HUMAN MGC72568 NF kappa B inhibitor epsilon NF kappa BIE NF-kappa-B inhibitor epsilon NF-kappa-BIE NFkappa BIE NFkappaB inhibitor epsilon NFKBIE Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor epsilon OTTHUMP00000016522 Slc35b2 solute carrier family 35, member B2 |
Images
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Fig1:
Western blot analysis of IKB epsilon on different lysates with Rabbit anti-IKB epsilon antibody (ET1705-40) at 1/1,000 dilution. Lane 1: THP-1 (Human acute monoblastic leukemia cell) cell lysate Lane 2: Jurkat (Human T-lymphoblastic cells) cell lysate Lane 3: HeLa (Human cervical adenocarcinoma cell) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 25 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1705-40, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 53 kDa Observed band size: 45 kDa |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IKB epsilon antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-40, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.