SKP1 Recombinant Rabbit Monoclonal Antibody [JM27-37]
cat.: ET1705-43
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat, Mouse |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JM27-37 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 19 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Skp1 aa 106-155 / 163. |
| Positive control: | PC-12 cell lysates, Hela, SH-SY5Y, human breast tissue. |
| Subcellular location: | Cytoplasm. Nucleus. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P FC |
1:500 1:50-1:100 1:50-1:100 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P63208 Human | Q9WTX5 Mouse | Q6PEC4 Rat |
| Alternative names: | Cyclin A/CDK2 associated p19 Cyclin A/CDK2 associated protein p19 Cyclin-A/CDK2-associated protein p19 EMC19 MGC34403 OCP 2 OCP II OCP II protein OCP-2 OCP-II OCP2 OCPII Organ of Corti protein 2 Organ of Corti protein II OTTHUMP00000159379 OTTHUMP00000159380 OTTHUMP00000228275 OTTHUMP00000228276 OTTHUMP00000228278 OTTHUMP00000228279 OTTHUMP00000228281 p19A p19skp1 RNA polymerase II elongation factor like protein RNA polymerase II elongation factor like protein OCP2 RNA polymerase II elongation factor-like protein S phase kinase associated protein 1 S phase kinase associated protein 1A S phase kinase associated protein 1A p19A S-phase kinase-associated protein 1 SIII Skp 1 SKP 1A skp1 SKP1_HUMAN SKP1A TCEB1L Transcription Elongation Factor B 1 like Transcription elongation factor B Transcription elongation factor B SIII polypeptide 1 like |
Images
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Fig1: ICC staining of SKP1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1705-43, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig2: ICC staining of SKP1 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1705-43, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-SKP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-43, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Flow cytometric analysis of SKP1 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1705-43, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig5:
Western blot analysis of SKP1 on different lysates with Rabbit anti-SKP1 antibody (ET1705-43) at 1/2,000 dilution. Lane 1: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate Exposure time: 20 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1705-43, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 18.7 kDa Observed band size: 19 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.