c-Rel Recombinant Rabbit Monoclonal Antibody [JM72-93]
cat.: ET1705-44
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P, IP
Clonality: Monoclonal
Clone number: JM72-93
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 69 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human c-Rel aa 501-550 / 619.
Positive control: Human tonsil tissue, human colon cancer tissue, human skin tissue, human placenta tissue, SKOV-3 cell lysate, Raji cell lysate, Daudi cell lysates, Jurkat cell lysate, A431 cell lysate.
Subcellular location: Cytoplasm. Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  IP

1:500
1:50-1:200
Use at an assay dependent concentration.
Uniprot #: SwissProt: Q04864 Human
Alternative names: Avian reticuloendotheliosis C REL C Rel protein c Rel proto oncogene protein Oncogene REL Oncogene REL avian reticuloendotheliosis Proto-oncogene c-Rel REL REL_HUMAN v rel avian reticuloendotheliosis viral oncogene homolog v rel reticuloendotheliosis viral oncogene homolog V rel reticuloendotheliosis viral oncogene homolog (avian)
Images
ET1705-44_1.jpg Fig1: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-c-Rel antibody. Counter stained with hematoxylin.
ET1705-44_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-c-Rel antibody. Counter stained with hematoxylin.
ET1705-44_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-c-Rel antibody. Counter stained with hematoxylin.
ET1705-44_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-c-Rel antibody. Counter stained with hematoxylin.
ET1705-44_5.jpg Fig5: Western blot analysis of c-Rel on different lysates with Rabbit anti-c-Rel antibody (ET1705-44) at 1/500 dilution.

Lane 1: SKOV-3 cell lysate
Lane 2: Raji cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 69 kDa
Observed band size: 75 kDa

Exposure time: 1 minute;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-44) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ET1705-44_6.jpg Fig6: Western blot analysis of c-Rel on Daudi cell lysates with Rabbit anti-c-Rel antibody (ET1705-44) at 1/500 dilution.

Lysates/proteins at 10 µg/Lane.

Predicted band size: 69 kDa
Observed band size: 75 kDa

Exposure time: 30 seconds;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-44) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ET1705-44_7.jpg Fig7: Western blot analysis of c-Rel on different lysates with Rabbit anti-c-Rel antibody (ET1705-44) at 1/500 dilution.

Lane 1: Jurkat cell lysate
Lane 2: A431 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 69 kDa
Observed band size: 75 kDa

Exposure time: 2 minutes;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-44) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.