c-Rel Recombinant Rabbit Monoclonal Antibody [JM72-93]
cat.: ET1705-44
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JM72-93 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 69 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human c-Rel aa 501-550 / 619. |
| Positive control: | HeLa cell lysate, K-562 cell lysate, Raji cell lysate, Daudi cell lysate, Jurkat cell lysate, A431 cell lysate, U-937 cell lysate, human tonsil tissue, human colon cancer tissue, human skin tissue, human placenta tissue. |
| Subcellular location: | Cytoplasm. Nucleus. |
| Recommended Dilutions:
WB IHC-P IP |
1:1,000 1:50-1:200 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q04864 Human |
| Alternative names: | Avian reticuloendotheliosis C REL C Rel protein c Rel proto oncogene protein Oncogene REL Oncogene REL avian reticuloendotheliosis Proto-oncogene c-Rel REL REL_HUMAN v rel avian reticuloendotheliosis viral oncogene homolog v rel reticuloendotheliosis viral oncogene homolog V rel reticuloendotheliosis viral oncogene homolog (avian) |
Images
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Fig1:
Western blot analysis of c-Rel on different lysates with Rabbit anti-c-Rel antibody (ET1705-44) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: K-562 cell lysate Lane 3: Raji cell lysate Lane 4: Daudi cell lysate Lane 5: Jurkat cell lysate Lane 6: A431 cell lysate Lane 7: U-937 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 69 kDa Observed band size: 75 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-44) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of c-Rel on different lysates with Rabbit anti-c-Rel antibody (ET1705-44) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-c-Rel KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 69 kDa Observed band size: 75 kDa Exposure time: 1 minute 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-44) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-c-Rel antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-c-Rel antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-c-Rel antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-c-Rel antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.