PI 3 Kinase p110 delta Recombinant Rabbit Monoclonal Antibody [JM83-24]
cat.: ET1705-46
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JM83-24 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 119 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human PI 3 Kinase p110 delta aa 197-246 / 1044. |
| Positive control: | Raji cell lysate, Jurkat cell lysate, RAW264.7 cell lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate, rat spleen tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200 |
| Uniprot #: | SwissProt: O00329 Human | O35904 Mouse | D4A5Q1 Rat |
| Alternative names: | 5-bisphosphate 3-kinase 110 kDa catalytic subunit delta 5-bisphosphate 3-kinase catalytic subunit delta isoform APDS GRB1 IMD14 p110d p110delta p110dp85a p85-ALPHA Phosphatidylinositol 3 kinase catalytic delta polypeptide Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit delta isoform Phosphatidylinositol 4,5 bisphosphate 3 kinase 110 kDa catalytic subunit delta Phosphatidylinositol 4,5 bisphosphate 3 kinase, catalytic subunit delta Phosphatidylinositol 45 bisphosphate 3 kinase catalytic subunit delta isoform Phosphatidylinositol-4 Phosphoinositide 3 kinase B Phosphoinositide 3 kinase C Phosphoinositide 3 kinase catalytic delta polypeptide Phosphoinositide 3 kinase, catalytic, delta polypeptide variant p37delta PI3 kinase p110 subunit delta PI3-kinase subunit delta PI3K PI3K-delta PI3Kdelta Pik3cd PIK3R1 PK3CD PK3CD_HUMAN PtdIns 3 kinase p110 PtdIns 3 kinase subunit p110 delta PtdIns-3-kinase subunit delta Ptd...... |
Images
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Fig1:
Western blot analysis of PI 3 Kinase p110 delta on different lysates with Rabbit anti-PI 3 Kinase p110 delta antibody (ET1705-46) at 1/1,000 dilution. Lane 1: Raji cell lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (20 µg/Lane) Lane 3: RAW264.7 cell lysate (20 µg/Lane) Lane 4: Mouse spleen tissue lysate (40 µg/Lane) Lane 5: Rat spleen tissue lysate (40 µg/Lane) Predicted band size: 119 kDa Observed band size: 105 kDa Exposure time: 12 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-46) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-PI 3 Kinase p110 delta antibody (ET1705-46) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-46) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.