PI 3 Kinase p110 delta Recombinant Rabbit Monoclonal Antibody [JM83-24]
cat.: ET1705-46
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JM83-24 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 119 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human PI 3 Kinase p110 delta aa 197-246 / 1044. |
| Positive control: | Mouse spleen tissue lysates, rat spleen tissue lysates, human tonsil tissue, human spleen tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:50-1:200 |
| Uniprot #: | SwissProt: O00329 Human | O35904 Mouse | D4A5Q1 Rat |
| Alternative names: | 5-bisphosphate 3-kinase 110 kDa catalytic subunit delta 5-bisphosphate 3-kinase catalytic subunit delta isoform APDS GRB1 IMD14 p110d p110delta p110dp85a p85-ALPHA Phosphatidylinositol 3 kinase catalytic delta polypeptide Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit delta isoform Phosphatidylinositol 4,5 bisphosphate 3 kinase 110 kDa catalytic subunit delta Phosphatidylinositol 4,5 bisphosphate 3 kinase, catalytic subunit delta Phosphatidylinositol 45 bisphosphate 3 kinase catalytic subunit delta isoform Phosphatidylinositol-4 Phosphoinositide 3 kinase B Phosphoinositide 3 kinase C Phosphoinositide 3 kinase catalytic delta polypeptide Phosphoinositide 3 kinase, catalytic, delta polypeptide variant p37delta PI3 kinase p110 subunit delta PI3-kinase subunit delta PI3K PI3K-delta PI3Kdelta Pik3cd PIK3R1 PK3CD PK3CD_HUMAN PtdIns 3 kinase p110 PtdIns 3 kinase subunit p110 delta PtdIns-3-kinase subunit delta Ptd...... |
Images
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Fig1:
Western blot analysis of PI 3 Kinase p110 delta on mouse spleen tissue lysates with Rabbit anti-PI 3 Kinase p110 delta antibody (ET1705-46) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 119 kDa Observed band size: 105 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-46) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2: Western blot analysis of PI 3 Kinase p110 delta on rat spleen tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-46, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PI 3 Kinase p110 delta antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-46, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PI 3 Kinase p110 delta antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-46, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.