alpha 1 Antichymotrypsin Recombinant Rabbit Monoclonal Antibody [JM83-29]
cat.: ET1705-47
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JM83-29 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 48 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein |
| Positive control: | Human serum, human tonsil tissue, human prostate tissue, human placenta tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P IP |
1:500-1:2,000 1:50-1:1,000 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P01011 Human |
| Alternative names: | SERPINA3 AACT AACT_HUMAN ACT Alpha-1-antichymotrypsin His-Pro-less Antichymotrypsin Cell growth inhibiting gene 24/25 protein Cell growth-inhibiting gene 24/25 protein GIG24 GIG25 Growth inhibiting protein 24 Growth inhibiting protein 25 MGC88254 Serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase, antitrypsin) member 3 Serine (or cysteine) proteinase inhibitor clade A member 3 Serine proteinase inhibitor clade A member 3 Serpin A3 Serpin family A member 3 Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 3 SERPINA3 |
Images
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Fig1: Western blot analysis of alpha 1 Antichymotrypsin on human serum using anti-alpha 1 Antichymotrypsin antibody at 1/1,000 dilution. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-alpha 1 Antichymotrypsin antibody. Counter stained with hematoxylin. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human prostate tissue with Rabbit anti-alpha 1 Antichymotrypsin antibody (ET1705-47) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-47) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-alpha 1 Antichymotrypsin antibody (ET1705-47) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-47) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.