ET1705-49

Id1 Recombinant Rabbit Monoclonal Antibody [JM92-13]

cat.: ET1705-49

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human
Applications:	WB, IF-Cell, IF-Tissue, IHC-P, FC
Clonality:	Monoclonal
Clone number:	JM92-13

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 16 kDa
Isotype:	IgG
Immunogen:	Synthetic peptide within Human Id1 aa 11-60 / 155.
Positive control:	293T cell lysates, Hela cell lysate, HepG2 cell lysate, A549, Hela, HepG2, human tonsil tissue, human liver tissue, human colon tissue, human placenta tissue.
Subcellular location:	Cytoplasm. Nucleus.
Recommended Dilutions: WB IF-Cell IF-Tissue IHC-P FC	1:500-1:2,000 1:50-1:200 1:50-1:200 1:50-1:200 1:50-1:100
Uniprot #:	SwissProt: P41134 Human
Alternative names:	bHLHb24 Class B basic helix-loop-helix protein 24 dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helix protein) DNA binding protein inhibitor ID 1 DNA binding protein inhibitor ID1 DNA-binding protein inhibitor ID-1 Dominant negative helix loop helix protein ID 1 ID ID1 ID1_HUMAN Inhibitor of Differentiation 1 Inhibitor of DNA binding 1 inhibitor of DNA binding 1, dominant negative helix-loop-helix protein

Images

Fig1: Western blot analysis of Id1 on 293T cell lysates with Rabbit anti-Id1 antibody (ET1705-49) at 1/500 dilution.

Lysates/proteins at 10 µg/Lane.

Predicted band size: 16 kDa
Observed band size: 22 kDa

Exposure time: 2 minutes;

15% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-49) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

Fig2: Western blot analysis of Id1 on different lysates with Rabbit anti-Id1 antibody (ET1705-49) at 1/500 dilution.

Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 16 kDa
Observed band size: 22 kDa

Exposure time: 1 minute;

15% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-49) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

	Fig3: ICC staining Id1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig4: Immunocytochemistry analysis of HeLa cells labeling Id1 with Rabbit anti-Id1 antibody (ET1705-49) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Id1 antibody (ET1705-49) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
	Fig5: ICC staining Id1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Id1 antibody. Counter stained with hematoxylin.

	Fig7: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Id1 antibody. Counter stained with hematoxylin.
	Fig8: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Id1 antibody. Counter stained with hematoxylin.
	Fig9: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Id1 antibody. Counter stained with hematoxylin.
	Fig10: Flow cytometric analysis of HepG2 cells with Id1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.