BMAL1 Recombinant Rabbit Monoclonal Antibody [JM17-34]
cat.: ET1705-5
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JM17-34 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 69 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human BMAL1 aa 550-599 / 626. |
| Positive control: | Hela cell lysate, NIH/3T3 cell lysate, rat brain tissue lysate, mouse spleen tissue lysate, Hela, SH-SY5Y. |
| Subcellular location: | Nucleus, PML body, Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell |
1:500-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: O00327 Human | Q9WTL8 Mouse | Q9EPW1 Rat |
| Alternative names: | ARNT like protein 1 brain and muscle Arntl Aryl hydrocarbon receptor nuclear translocator like Aryl hydrocarbon receptor nuclear translocator like protein 1 Aryl hydrocarbon receptor nuclear translocator-like protein 1 Basic helix loop helix PAS orphan MOP3 Basic helix loop helix PAS protein MOP3 Basic-helix-loop-helix-PAS protein MOP3 bHLH PAS protein JAP3 bHLH-PAS protein JAP3 bHLHe5 BMAL 1 BMAL1_HUMAN BMAL1c Brain and muscle ARNT like 1 Brain and muscle ARNT-like 1 CG8727 PA Class E basic helix-loop-helix protein 5 cycle JAP 3 JAP3 Member of PAS protein 3 Member of PAS superfamily 3 MGC47515 MOP 3 MOP3 PAS domain-containing protein 3 PASD 3 PASD3 TIC |
Images
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Fig1: ICC staining of BMAL1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1705-5, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig2: ICC staining of BMAL1 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1705-5, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
|
Fig3:
Western blot analysis of BMAL1 on different lysates with Rabbit anti-BMAL1 antibody (ET1705-5) at 1/1,000 dilution. Lane 1: MCF7(Human breast cancer cells) cell lysatel) Exposure time: 17 seconds ; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1705-5, 1/1,000 in primary antibody dilution buffer 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 68.8 kDa Observed band size: 69 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.