GABARAP Recombinant Rabbit Monoclonal Antibody [JM30-30]
cat.: ET1705-53
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JM30-30 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 14 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human GABARAP aa 10-59 / 117. |
| Positive control: | HeLa cell lysate, SH-SY5Y cell lysate, mouse kidney tissue lysate, rat liver tissue lysate, human placenta tissue, human kidney tissue, human pancreas tissue. |
| Subcellular location: | Endomembrane system. Cytoplasm, cytoskeleton. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: O95166 Human | Q8R3R8 Mouse | P60517 Rat |
| Alternative names: | ATG8A FLC 3B FLC3B FLJ25768 GABA type A receptor associated protein GABA(A) receptor associated protein GABA(A) receptor-associated protein GABARAP a GABARAP Gamma aminobutyric acid receptor associated protein Gamma-aminobutyric acid receptor-associated protein GBRAP_HUMAN MGC120154 MGC120155 MM 46 MM46 |
Images
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Fig1:
Western blot analysis of GABARAP on different lysates with Rabbit anti-GABARAP antibody (ET1705-53) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: SH-SY5Y cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 14 kDa Observed band size: 16 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-53) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of GABARAP on mouse kidney tissue lysate with Rabbit anti-GABARAP antibody (ET1705-53) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 20 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1705-53, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/100,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 13.9 kDa Observed band size: 16 kDa |
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Fig3:
Western blot analysis of GABARAP on rat liver tissue lysate with Rabbit anti-GABARAP antibody (ET1705-53) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 20 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1705-53, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/100,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 13.9 kDa Observed band size: 16 kDa |
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Fig4: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-GABARAP antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-53, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-GABARAP antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-53, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-GABARAP antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-53, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.