GABARAP Recombinant Rabbit Monoclonal Antibody [JM30-30]
cat.: ET1705-53
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: JM30-30
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 14 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human GABARAP aa 10-59 / 117.
Positive control: Mouse kidney tissue lysate, rat liver tissue lysate, human placenta tissue, human kidney tissue, human pancreas tissue, Hela.
Subcellular location: Endomembrane system. Cytoplasm, cytoskeleton.
Recommended Dilutions:
  WB
  IHC-P
  FC

1:500
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: O95166 Human | Q8R3R8 Mouse | P60517 Rat
Alternative names: ATG8A FLC 3B FLC3B FLJ25768 GABA type A receptor associated protein GABA(A) receptor associated protein GABA(A) receptor-associated protein GABARAP a GABARAP Gamma aminobutyric acid receptor associated protein Gamma-aminobutyric acid receptor-associated protein GBRAP_HUMAN MGC120154 MGC120155 MM 46 MM46
Images
ET1705-53_1.jpg Fig1: Western blot analysis of GABARAP on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-53, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Mouse kidney tissue lysate
Lane 2: Rat liver tissue lysate
ET1705-53_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-GABARAP antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-53, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-53_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-GABARAP antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-53, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-53_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-GABARAP antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-53, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-53_5.jpg Fig5: Flow cytometric analysis of GABARAP was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1705-53, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.