Ferritin Light Chain Recombinant Rabbit Monoclonal Antibody [JM10-37]
cat.: ET1705-54
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IP
Clonality: Monoclonal
Clone number: JM10-37
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 20 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Ferritin Light Chain aa 126-175 / 175.
Positive control: Rat liver tissue lysates, human liver tissue, human brain tissue, human kidney tissue, mouse testis tissue, mouse kidney tissue.
Subcellular location: Cytosol, extracellular exosome, extracellular region, autolysosome, azurophil granule lumen, cytoplasm, intracellular ferritin complex, membrane.
Recommended Dilutions:
  WB
  IHC-P
  IP

1:1,000-1:5,000
1:100-1:500
Use at an assay dependent concentration.
Uniprot #: SwissProt: P02792 Human | P29391 Mouse | P02793 Rat
Alternative names: Ferritin L chain Ferritin L subunit Ferritin light chain Ferritin light polypeptide ferritin light polypeptide like 3 FRIL_HUMAN FTL LFTD NBIA 3 NBIA3
Images
ET1705-54_1.jpg Fig1: Western blot analysis of Ferritin Light Chain on rat liver tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-54, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET1705-54_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Ferritin Light Chain antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-54, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-54_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-Ferritin Light Chain antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-54, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-54_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Ferritin Light Chain antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-54, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-54_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Ferritin Light Chain antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-54, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-54_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Ferritin Light Chain antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-54, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.