Ferritin Light Chain Recombinant Rabbit Monoclonal Antibody [JM10-37]
cat.: ET1705-54
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | JM10-37 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 20 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Ferritin Light Chain aa 126-175 / 175. |
| Positive control: | A549 cell lysate, human liver tissue lysate, mouse testis tissue lysate, mouse liver tissue lysate, rat liver tissue lysate. |
| Subcellular location: | Cytosol, extracellular exosome, extracellular region, autolysosome, azurophil granule lumen, cytoplasm, intracellular ferritin complex, membrane. |
| Recommended Dilutions:
WB IP |
1:1,000-1:5,000 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P02792 Human | P29391 Mouse | P02793 Rat |
| Alternative names: | Ferritin L chain Ferritin L subunit Ferritin light chain Ferritin light polypeptide ferritin light polypeptide like 3 FRIL_HUMAN FTL LFTD NBIA 3 NBIA3 Ftl1 |
Images
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Fig1:
Western blot analysis of Ferritin Light Chain on different lysates with Rabbit anti-Ferritin Light Chain antibody (ET1705-54) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 µg/Lane) Lane 2: Human liver tissue lysate (40 µg/Lane) Lane 3: Mouse testis tissue lysate (40 µg/Lane) Lane 4: Mouse liver tissue lysate (40 µg/Lane) Lane 5: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 20 kDa Observed band size: 20 kDa Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-54) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.