ENO1 Recombinant Rabbit Monoclonal Antibody [JM63-53]
cat.: ET1705-56
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Zebrafish |
| Applications: | WB, FC, IF-Cell, IF-Tissue, IP |
| Clonality: | Monoclonal |
| Clone number: | JM63-53 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 47 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human ENO1 aa 1-50 / 434. |
| Positive control: | Rat brain tissue lysate, NIH-3T3 cell lysate, Zebrafish tissue lysates, HepG2 cell lysate, Hela cell lysate, MCF-7. |
| Subcellular location: | Cell membrane, Cytoplasm, Membrane, Nucleus. |
| Recommended Dilutions:
WB FC IF-Cell IF-Tissue IP |
1:500-1:2,000 1:50-1:100 1:10-1:50 1:10-1:50 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P06733 Human | P17182 Mouse | P04764 Rat |
| Alternative names: | 2 phospho D glycerate hydro lyase 2-phospho-D-glycerate hydro-lyase Alpha enolase Alpha enolase like 1 Alpha-enolase C myc promoter binding protein C-myc promoter-binding protein EC 4.2.1.11 eno1 ENO1L1 ENOA_HUMAN Enolase 1 (alpha) Enolase 1 (alpha) like 1 Enolase 1 Enolase alpha MBP 1 MBP-1 MBP1 MBPB1 MPB 1 MPB-1 MPB1 MYC promoter binding protein 1 NNE Non neural enolase Non-neural enolase Phosphopyruvate hydratase Plasminogen binding protein Plasminogen-binding protein PPH Tau crystallin |
Images
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Fig1:
Western blot analysis of ENO1 on different lysates using anti-ENO1 antibody at 1/1,000 dilution. Positive control: Lane 1: Rat brain tissue lysate Lane 2: NIH-3T3 cell lysate Lane 3: Hela cell lysate Lane 4: HepG2 cell lysate |
|
Fig2: Western blot analysis of ENO1 on Zebrafish tissue lysates using anti-ENO1 antibody at 1/1,000 dilution. |
|
Fig3: Flow cytometric analysis of MCF-7 cells with ENO1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.