ENO1 Recombinant Rabbit Monoclonal Antibody [JM63-53]
cat.: ET1705-56
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Zebrafish |
| Applications: | WB, FC, IF-Tissue, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JM63-53 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 47 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human ENO1 aa 1-50 / 434. |
| Positive control: | HeLa cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, C6 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, Zebrafish tissue lysate, human lung cancer tissue, HeLa, NIH/3T3. |
| Subcellular location: | Cell membrane, Cytoplasm, Membrane, Nucleus. |
| Recommended Dilutions:
WB FC IF-Tissue IHC-P IF-Cell |
1:2,000 1:1,000 1:50-1:200 1:1,000 1:100 |
| Uniprot #: | SwissProt: P06733 Human | P17182 Mouse | P04764 Rat |
| Alternative names: | 2 phospho D glycerate hydro lyase 2-phospho-D-glycerate hydro-lyase Alpha enolase Alpha enolase like 1 Alpha-enolase C myc promoter binding protein C-myc promoter-binding protein EC 4.2.1.11 eno1 ENO1L1 ENOA_HUMAN Enolase 1 (alpha) Enolase 1 (alpha) like 1 Enolase 1 Enolase alpha MBP 1 MBP-1 MBP1 MBPB1 MPB 1 MPB-1 MPB1 MYC promoter binding protein 1 NNE Non neural enolase Non-neural enolase Phosphopyruvate hydratase Plasminogen binding protein Plasminogen-binding protein PPH Tau crystallin |
Images
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Fig1:
Western blot analysis of ENO1 on different lysates with Rabbit anti-ENO1 antibody (ET1705-56) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (20 µg/Lane) Lane 3: NIH/3T3 cell lysate (20 µg/Lane) Lane 4: C6 cell lysate (20 µg/Lane) Lane 5: Mouse brain tissue lysate (20 µg/Lane) Lane 6: Rat brain tissue lysate (20 µg/Lane) Lane 7: Zebrafish tissue lysate (20 µg/Lane) Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-56) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-ENO1 antibody (ET1705-56) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-56) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunocytochemistry analysis of HeLa cells labeling ENO1 with Rabbit anti-ENO1 antibody (ET1705-56) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ENO1 antibody (ET1705-56) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig4:
Immunocytochemistry analysis of NIH/3T3 cells labeling ENO1 with Rabbit anti-ENO1 antibody (ET1705-56) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ENO1 antibody (ET1705-56) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig5:
Flow cytometric analysis of HeLa cells labeling ENO1. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1705-56, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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