Pyruvate Dehydrogenase E1 beta subunit Recombinant Rabbit Monoclonal Antibody [JM66-33]
cat.: ET1705-57
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC, IP
Clonality: Monoclonal
Clone number: JM66-33
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 39 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Pyruvate Dehydrogenase E1 beta subunit aa 310-359 / 359.
Positive control: HepG2, Hela, PC-12, mouse kidney, rat heart tissue, human kidney tissue, human stomach cancer tissue, mouse hippocampus tissue, mouse brain tissue.
Subcellular location: Mitochondrion matrix.
Recommended Dilutions:
  WB
  IHC-P
  FC
  IP

1:500-1:2,000
1:50-1:200
1:50-1:100
Use at an assay dependent concentration.
Uniprot #: SwissProt: P11177 Human | Q9D051 Mouse | P49432 Rat
Alternative names: DKFZp564K0164 mitochondrial ODPB_HUMAN pdhB PDHBD PDHE1 B PDHE1-B PHE1B Pyruvate dehydrogenase (lipoamide) beta Pyruvate dehydrogenase E1 beta polypeptide Pyruvate dehydrogenase E1 component subunit beta Pyruvate dehydrogenase E1 component subunit beta mitochondrial
Images
ET1705-57_1.jpg Fig1: Western blot analysis of Pyruvate Dehydrogenase E1 beta subunit on HepG2 cell lysate(1) and Hela cell lysate(2) using anti-Pyruvate Dehydrogenase E1 beta subunit antibody at 1/1,000 dilution.
ET1705-57_2.jpg Fig2: Western blot analysis of Pyruvate Dehydrogenase E1 beta subunit on mouse kidney tissue lysate(1) and PC-12 cell lysate(2) using anti-Pyruvate Dehydrogenase E1 beta subunit antibody at 1/1,000 dilution.
ET1705-57_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Pyruvate Dehydrogenase E1 beta subunit antibody. Counter stained with hematoxylin.
ET1705-57_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Pyruvate Dehydrogenase E1 beta subunit antibody. Counter stained with hematoxylin.
ET1705-57_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Pyruvate Dehydrogenase E1 beta subunit antibody. Counter stained with hematoxylin.
ET1705-57_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Pyruvate Dehydrogenase E1 beta subunit antibody. Counter stained with hematoxylin.
ET1705-57_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue using anti-Pyruvate Dehydrogenase E1 beta subunit antibody. Counter stained with hematoxylin.
ET1705-57_8.jpg Fig8: Flow cytometric analysis of Hela cells with Pyruvate Dehydrogenase E1 beta subunit antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.