Pyruvate Dehydrogenase E1 beta subunit Recombinant Rabbit Monoclonal Antibody [JM66-33]
cat.: ET1705-57
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | JM66-33 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 39 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Pyruvate Dehydrogenase E1 beta subunit aa 310-359 / 359. |
| Positive control: | HepG2, Hela, PC-12, mouse kidney, rat heart tissue, human kidney tissue, human stomach cancer tissue, mouse hippocampus tissue, mouse brain tissue. |
| Subcellular location: | Mitochondrion matrix. |
| Recommended Dilutions:
WB IHC-P FC IP |
1:500-1:2,000 1:50-1:200 1:50-1:100 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P11177 Human | Q9D051 Mouse | P49432 Rat |
| Alternative names: | DKFZp564K0164 mitochondrial ODPB_HUMAN pdhB PDHBD PDHE1 B PDHE1-B PHE1B Pyruvate dehydrogenase (lipoamide) beta Pyruvate dehydrogenase E1 beta polypeptide Pyruvate dehydrogenase E1 component subunit beta Pyruvate dehydrogenase E1 component subunit beta mitochondrial |
Images
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Fig1: Western blot analysis of Pyruvate Dehydrogenase E1 beta subunit on HepG2 cell lysate(1) and Hela cell lysate(2) using anti-Pyruvate Dehydrogenase E1 beta subunit antibody at 1/1,000 dilution. |
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Fig2: Western blot analysis of Pyruvate Dehydrogenase E1 beta subunit on mouse kidney tissue lysate(1) and PC-12 cell lysate(2) using anti-Pyruvate Dehydrogenase E1 beta subunit antibody at 1/1,000 dilution. |
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Fig3: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Pyruvate Dehydrogenase E1 beta subunit antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Pyruvate Dehydrogenase E1 beta subunit antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Pyruvate Dehydrogenase E1 beta subunit antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Pyruvate Dehydrogenase E1 beta subunit antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue using anti-Pyruvate Dehydrogenase E1 beta subunit antibody. Counter stained with hematoxylin. |
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Fig8: Flow cytometric analysis of Hela cells with Pyruvate Dehydrogenase E1 beta subunit antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.