SMYD3 Recombinant Rabbit Monoclonal Antibody [JM73-63]
cat.: ET1705-58
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | JM73-63 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 49 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human SMYD3 aa 251-300 / 428. |
| Positive control: | Rat kidney tissue lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate, PC-12 cell lysate, NIH-3T3 cell lysate, HepG2, Hela cell lysate, LOVO, Hela, human tonsil tissue, human colon cancer tissue, human placenta tissue, mouse stomach tissue. |
| Subcellular location: | Cytoplasm. Nucleus. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P FC IP |
1:500-1:2,000 1:50-1:200 1:50-1:200 1:50-1:200 1:50-1:100 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q9H7B4 Human | Q9CWR2 Mouse |
| Alternative names: | bA74P14.1 (novel protein) bA74P14.1 FLJ21080 histone lysine N methyltransferase SMYD3 KMT3E MGC104324 SET and MYND domain containing 3 SET and MYND domain containing protein 3 SET and MYND domain-containing protein 3 SMYD 3 Smyd3 SMYD3 protein SMYD3_HUMAN Zinc finger MYND domain containing 1 Zinc finger MYND domain containing protein 1 Zinc finger MYND domain-containing protein 1 Zinc finger protein subfamily 3A MYND domain containing 1 Zinc finger protein, subfamily 3A (MYND domain containing), 1 ZMYND 1 ZMYND1 ZNFN3A1 |
Images
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Fig1:
Western blot analysis of SMYD3 on different lysates with Rabbit anti-SMYD3 antibody (ET1705-58) at 1/1000 dilution. Lane 1: Rat kidney tissue lysate Lane 2: PC-12 cell lysate Lane 3: Hela cell lysate Lane 4: Mouse spleen tissue lysate Lane 5: NIH-3T3 cell lysate Lane 6: Rat spleen tissue lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 39 kDa Observed band size: 39 kDa Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-58) at 1/1000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
"Immunocytochemistry analysis of Hela cells labeling SMYD3 with Rabbit anti-SMYD3 antibody (ET1705-58) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SMYD3 antibody (ET1705-58) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI." |
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Fig3:
Immunocytochemistry analysis of HepG2 cells labeling SMYD3 with Rabbit anti-SMYD3 antibody (ET1705-58) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SMYD3 antibody (ET1705-58) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig4:
Immunocytochemistry analysis of LOVO cells labeling SMYD3 with Rabbit anti-SMYD3 antibody (ET1705-58) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SMYD3 antibody (ET1705-58) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SMYD3 antibody. Counter stained with hematoxylin. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-SMYD3 antibody (ET1705-58) at 1/50 dilution.Hela cells The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-58) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-SMYD3 antibody (ET1705-58) at 1/50 dilution.Hela cells The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-58) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig8:
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue with Rabbit anti-SMYD3 antibody (ET1705-58) at 1/50 dilution.Hela cells The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-58) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig9: Flow cytometric analysis of Hela cells with SMYD3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.