SMYD3 Recombinant Rabbit Monoclonal Antibody [JM73-63]
cat.: ET1705-58
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P, FC, IP
Clonality: Monoclonal
Clone number: JM73-63
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 49 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human SMYD3 aa 251-300 / 428.
Positive control: MCF7 cell lysate, HCT 116 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate, Hela, HepG2, LOVO, human tonsil tissue, human colon cancer tissue, human placenta tissue, mouse stomach tissue.
Subcellular location: Cytoplasm. Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
  FC
  IP
1:500-1:2,000
1:50-1:200
1:50-1:200
1:50-1:200
1:50-1:100
Use at an assay dependent concentration.
Uniprot #: SwissProt: Q9H7B4 Human | Q9CWR2 Mouse
Alternative names: bA74P14.1 (novel protein) bA74P14.1 FLJ21080 histone lysine N methyltransferase SMYD3 KMT3E MGC104324 SET and MYND domain containing 3 SET and MYND domain containing protein 3 SET and MYND domain-containing protein 3 SMYD 3 Smyd3 SMYD3 protein SMYD3_HUMAN Zinc finger MYND domain containing 1 Zinc finger MYND domain containing protein 1 Zinc finger MYND domain-containing protein 1 Zinc finger protein subfamily 3A MYND domain containing 1 Zinc finger protein, subfamily 3A (MYND domain containing), 1 ZMYND 1 ZMYND1 ZNFN3A1
Images
ET1705-58_1.jpg Fig1: Western blot analysis of SMYD3 on different lysates with Rabbit anti-SMYD3 antibody (ET1705-58) at 1/2,000 dilution.

Lane 1: MCF7 cell lysate (10 µg/Lane)
Lane 2: HCT 116 cell lysate (10 µg/Lane)
Lane 3: NIH/3T3 cell lysate (10 µg/Lane)
Lane 4: PC-12 cell lysate (10 µg/Lane)
Lane 5: Mouse spleen tissue lysate (20 µg/Lane)
Lane 6: Rat spleen tissue lysate (20 µg/Lane)

Predicted band size: 49 kDa
Observed band size: 40 kDa

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-58) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1705-58_2.jpg Fig2: Immunocytochemistry analysis of Hela cells labeling SMYD3 with Rabbit anti-SMYD3 antibody (ET1705-58) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SMYD3 antibody (ET1705-58) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
ET1705-58_3.jpg Fig3: Immunocytochemistry analysis of HepG2 cells labeling SMYD3 with Rabbit anti-SMYD3 antibody (ET1705-58) at 1/200 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SMYD3 antibody (ET1705-58) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
ET1705-58_4.jpg Fig4: Immunocytochemistry analysis of LOVO cells labeling SMYD3 with Rabbit anti-SMYD3 antibody (ET1705-58) at 1/200 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SMYD3 antibody (ET1705-58) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI..
ET1705-58_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SMYD3 antibody. Counter stained with hematoxylin.
ET1705-58_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-SMYD3 antibody (ET1705-58) at 1/50 dilution.Hela cells
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-58) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-58_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-SMYD3 antibody (ET1705-58) at 1/50 dilution.Hela cells
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-58) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-58_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue with Rabbit anti-SMYD3 antibody (ET1705-58) at 1/50 dilution.Hela cells
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-58) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-58_9.jpg Fig9: Flow cytometric analysis of Hela cells with SMYD3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.