Angiopoietin 2 Recombinant Rabbit Monoclonal Antibody [JM71-34]
cat.: ET1705-6
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JM71-34
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 57 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Angiopoietin 2 aa 351-400 / 496.
Positive control: TF-1 cell lysate, human liver tissue lysate, human placenta tissue lysate, mouse liver tissue lysate, human colon carcinoma tissue, human placenta tissue, mouse liver tissue.
Subcellular location: Secreted.
Recommended Dilutions:
  WB
  IHC-P

1:500-1:2,000
1:50-1:200
Uniprot #: SwissProt: O15123 Human | O35608 Mouse | O35462 Rat
Alternative names: AGPT 2 Agpt2 ANG 2 ANG-2 ANG2 Angiopoietin 2a Angiopoietin 2B Angiopoietin-2 Angiopoietin2 ANGP2_HUMAN ANGPT 2 Angpt2 Tie2 ligand
Images
ET1705-6_1.jpg Fig1: Western blot analysis of Angiopoietin 2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-6, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: TF-1 cell lysate
Lane 2: human liver tissue lysate
Lane 3: human placenta tissue lysate
Lane 4: mouse liver tissue lysate
ET1705-6_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Angiopoietin 2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-6, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-6_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Angiopoietin 2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-6, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-6_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Angiopoietin 2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-6, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.