Angiopoietin 2 Recombinant Rabbit Monoclonal Antibody [JM71-34]
cat.: ET1705-6
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JM71-34 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 57 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Angiopoietin 2 aa 351-400 / 496. |
| Positive control: | TF-1 cell lysate, human liver tissue lysate, human placenta tissue lysate, mouse liver tissue lysate, human colon carcinoma tissue, human placenta tissue, mouse liver tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:2,000 1:50-1:200 1μg/mL |
| Uniprot #: | SwissProt: O15123 Human | O35608 Mouse | O35462 Rat |
| Alternative names: | AGPT 2 Agpt2 ANG 2 ANG-2 ANG2 Angiopoietin 2a Angiopoietin 2B Angiopoietin-2 Angiopoietin2 ANGP2_HUMAN ANGPT 2 Angpt2 Tie2 ligand |
Images
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Fig1:
Western blot analysis of Angiopoietin 2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-6, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: TF-1 cell lysate Lane 2: human liver tissue lysate Lane 3: human placenta tissue lysate Lane 4: mouse liver tissue lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Angiopoietin 2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-6, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Angiopoietin 2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-6, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Angiopoietin 2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-6, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Flow cytometric analysis of TF-1 cells labeling Angiopoietin 2. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1705-6, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.