WDR5 Recombinant Rabbit Monoclonal Antibody [JM73-53]
cat.: ET1705-60
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Cell
Clonality: Monoclonal
Clone number: JM73-53
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 37 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human WDR5 aa 285-334 / 334.
Positive control: HeLa cell lysate, Saos-2 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate, human breast carcinoma tissue, human colon carcinoma tissue, human thyroid tissue, mouse brain tissue, rat brain tissue, PC-12.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
1:1,000
1:50-1:200
1:50
Uniprot #: SwissProt: P61964 Human | P61965 Mouse | Q498M4 Rat
Alternative names: 2410008O07Rik AA408785 AA960360 BIG 3 Big BIG3 BMP2 induced 3 kb gene protein BMP2 induced gene, 3-KB BMP2-induced 3-kb gene protein MGC114572 OTTHUMP00000162494 SWD 3 SWD3 SWD3 Set1c WD40 repeat protein homolog WD repeat containing protein 5 WD repeat domain 5 WD repeat protein 5 WD repeat-containing protein 5 WD repeat-containing protein BIG-3 WDR 5 Wdr5 WDR5_HUMAN
Images
ET1705-60_1.jpg Fig1: Western blot analysis of WDR5 on different lysates with Rabbit anti-WDR5 antibody (ET1705-60) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: Saos-2 cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: RAW264.7 cell lysate
Lane 5: PC-12 cell lysate
Lane 6: Mouse spleen tissue lysate
Lane 7: Rat spleen tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 37 kDa
Observed band size: 37 kDa

Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-60) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1705-60_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-WDR5 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-60, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-60_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-WDR5 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-60, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-60_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-WDR5 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-60, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-60_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-WDR5 antibody (ET1705-60) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-60) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-60_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-WDR5 antibody (ET1705-60) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-60) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-60_7.jpg Fig7: Immunocytochemistry analysis of PC-12 cells labeling WDR5 with Rabbit anti-WDR5 antibody (ET1705-60) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-WDR5 antibody (ET1705-60) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.