KPNA2 Recombinant Rabbit Monoclonal Antibody [JM90-33]
cat.: ET1705-61
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JM90-33 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 58 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human KPNA2 aa 41-90 / 529. |
| Positive control: | Hela cell lysate, 293T cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, 293T. |
| Subcellular location: | Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Membrane, Nucleus. |
| Recommended Dilutions:
WB FC |
1:500-1:2,000 1:50-1:100 |
| Uniprot #: | SwissProt: P52292 Human | P52293 Mouse | Q6P6T9 Rat |
| Alternative names: | IMA1_HUMAN Importin alpha 1 Importin alpha 2 Importin alpha 2 subunit Importin alpha P1 Importin subunit alpha-1 IPO A1 IPOA 1 IPOA1 Karyopherin alpha 2 Karyopherin alpha 2 RAG cohort 1 Karyopherin alpha 2 subunit Karyopherin subunit alpha-2 KPNA2 KPNA2 protein Pendulin QIP 2 QIP2 RAG cohort 1 RAG cohort protein 1 RCH 1 RCH1 SRP 1 SRP1 alpha SRP1 SRP1-alpha SRP1alpha |
Images
|
Fig1:
Western blot analysis of KPNA2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-61, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: 293T cell lysate Lane 3: HepG2 cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: PC-12 cell lysate |
|
Fig2: Flow cytometric analysis of KPNA2 was done on 293T cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1705-61, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.