RRM2 Recombinant Rabbit Monoclonal Antibody [JM93-43]
cat.: ET1705-62
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP, IF-Cell, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JM93-43 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human RRM2 aa 1-147 / 389. |
| Positive control: | HeLa cell lysate, HDLM-2 cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate, HeLa, human tonsil tissue, human appendix tissue. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IP IHC-P |
1:500-1:2,000 1:100 1:20-1:50 1-2μg/sample 1:200-1:1,000 |
| Uniprot #: | SwissProt: P31350 Human | P11157 Mouse | Q4KLN6 Rat |
| Alternative names: | cb111 chunp6884 etID309896.20 R2 reductase M2 polypeptide variant 1 reductase M2 polypeptide variant 2 reductase M2 polypeptide variant 3a reductase M2 polypeptide variant 3b reductase M2 polypeptide variant 3c reductase M2 polypeptide variant 3d Ribonucleoside-diphosphate reductase subunit M2 Ribonucleotide reductase M2 Ribonucleotide reductase M2 polypeptide Ribonucleotide reductase M2 subunit ribonucleotide reductase protein r2 class I Ribonucleotide reductase regulatory subunit M2 Ribonucleotide reductase small chain Ribonucleotide reductase small subunit Ribonucleotide reductase, R2 subunit RIR2_HUMAN RR2 RR2M RRM2 |
Images
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Fig1:
Western blot analysis of RRM2 on different lysates with Rabbit anti-RRM2 antibody (ET1705-62) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HDLM-2 cell lysate Lane 3: Mouse liver tissue lysate Lane 4: Rat liver tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-62) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HeLa cells labeling RRM2 with Rabbit anti-RRM2 antibody (ET1705-62) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-RRM2 antibody (ET1705-62) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-RRM2 antibody (ET1705-62) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-62) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-RRM2 antibody (ET1705-62) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-62) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.