PGC1 beta Recombinant Rabbit Monoclonal Antibody [JM33-24]
cat.: ET1705-65
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | JM33-24 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 113 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human PGC1 beta aa 974-1023 / 1023. |
| Positive control: | SK-Br-3 cell lysate, HeLa cell lysate, HepG2 cell lysate, rat spleen tissue lysates, HL-60. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB FC IP |
1:500-1:2,000 1:50-1:200 1:10-1:50 |
| Uniprot #: | SwissProt: Q86YN6 Human | Q811R2 Rat |
| Alternative names: | PERC peroxisome proliferative activated receptor, gamma, coactivator 1, peroxisome proliferator-activated receptor gamma coactivator 1 beta Peroxisome proliferator-activated receptor gamma coactivator 1-beta peroxisome proliferator-activated receptor gamma, coactivator 1 beta PGC-1(beta) PGC-1-beta PGC-1-related estrogen receptor alpha coactivator PGC1 PPAR gamma coactivator-1beta PPAR-gamma coactivator 1-beta PPARGC-1-beta PPARGC1 Ppargc1b PRGC2_HUMAN |
Images
|
Fig1:
Western blot analysis of PGC1 beta on different lysates with Rabbit anti-PGC1 beta antibody (ET1705-65) at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: HeLa cell lysate Lane 3: HepG2 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 113 kDa Observed band size: 113 kDa Exposure time: 24 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-65) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Western blot analysis of PGC1 beta on rat spleen tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-65, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig3: Flow cytometric analysis of PGC1 beta was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1705-65, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.