Cyclophilin F Recombinant Rabbit Monoclonal Antibody [JM71-39]
cat.: ET1705-7
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | JM71-39 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 22 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Cyclophilin F aa 30-79 / 207. |
| Positive control: | HEK-293 cell lysate, HeLa cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate, human colon carcinoma tissue, human breast carcinoma tissue, mouse brain tissue, human colon tissue, HeLa. |
| Subcellular location: | Mitochondrion matrix. |
| Recommended Dilutions:
WB IHC-P IP FC |
1:500-1:2,000 1:50-1:400 Use at an assay dependent concentration. 1:1,000 |
| Uniprot #: | SwissProt: P30405 Human | Q99KR7 Mouse | P29117 Rat |
| Alternative names: | Cyclophilin 3 cyclophilin D Cyclophilin F Cyp D CyP M CyP-D CyP-M CYP3 CypD hCyP3 mitochondrial Mitochondrial cyclophilin Peptidyl prolyl cis trans isomerase mitochondral Peptidyl-prolyl cis-trans isomerase F Peptidyl-prolyl cis-trans isomerase F mitochondrial Peptidylprolyl isomerase F (cyclophilin F) Peptidylprolyl isomerase F PPIase PPIase F Ppif PPIF_HUMAN Rotamase Rotamase F |
Images
|
Fig1:
Western blot analysis of Cyclophilin F on different lysates with Rabbit anti-Cyclophilin F antibody (ET1705-7) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: Mouse liver tissue lysate (40 µg/Lane) Lane 4: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 22 kDa Observed band size: 17 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-7) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Cyclophilin F antibody (ET1705-7) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-7) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Cyclophilin F antibody (ET1705-7) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-7) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Cyclophilin F antibody (ET1705-7) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-7) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Cyclophilin F antibody (ET1705-7) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-7) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6:
Flow cytometric analysis of HeLa cells labeling Cyclophilin F. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1705-7, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.