DDAH1 Recombinant Rabbit Monoclonal Antibody [JM53-18]
cat.: ET1705-71
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | JM53-18 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 31 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human DDAH1 aa 100-285 / 285. |
| Positive control: | SH-SY5Y cell lysate, HepG2 cell lysate, HUVEC cell lysate, human prostate tissue, human kidney tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IP |
1:5,000 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: O94760 Human |
| Alternative names: | DDAH DDAH I DDAH-1 DDAH1 DDAH1_HUMAN DDAHI Dimethylargininase 1 Dimethylargininase-1 Dimethylarginine dimethylaminohydrolase 1 N(G) N(G)-dimethylarginine dimethylaminohydrolase 1 NG NG dimethylarginine dimethylaminohydrolase |
Images
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Fig1:
Western blot analysis of DDAH1 on different lysates with Rabbit anti-DDAH1 antibody (ET1705-71) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-DDAH1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 31 kDa Observed band size: 37 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-71) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of DDAH1 on different lysates with Rabbit anti-DDAH1 antibody (ET1705-71) at 1/5,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: HepG2 cell lysate Lane 3: HUVEC cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 31 kDa Observed band size: 37 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-71) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.